Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 4H9] (IgG2a) to Human CENPF / CENP-F
IHC, Western blot, Immunoprecipitation
Human CENPF / CENP-F
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [4H9]
Protein G purified
Specificity and Use
CENPF / CENP-F antibody was raised against gST fusion protein expressed in E. coli corresponding to aa 1759-2093 of human mitosin.
mitosin, a 350 kD phosphoprotein that is specifically involved in mitotic-phase progression. Mitosin is expressed throughout S, G2, and M phases of the cell cycle, but is absent in G0 and G1. It associates with the mitotic apparatus during M-phase and is redistributed from the nucleus to the centromere, spindle, and midbody during M-phase progression. Its usefulness as a proliferation marker is under active investigation
CENPF Antibody, AH antigen Antibody, Centromere protein F Antibody, CENF Antibody, CENP-F Antibody, Kinetochore protein CENPF Antibody, Mitosin Antibody, CENP-F kinetochore protein Antibody, Hcp-1 Antibody, PRO1779 Antibody
Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation.