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Anti-CDYL Antibody (aa586-598) LS-C34652

Note: This antibody replaces LS-C79046

Ordering

Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular CDYL Antibodies

Anti-CDYL Antibody (aa6-34, AP) LS-C247003
Rabbit Polyclonal (IgG) to Human CDYL
Human
Western blot, ELISA
AP Conjugated
Anti-CDYL Antibody (aa6-34, FITC) LS-C247006
Rabbit Polyclonal (IgG) to Human CDYL
Human
Western blot, ELISA
FITC Conjugated
Anti-CDYL Antibody (aa6-34, Azide-free, HRP) LS-C249647
Rabbit Polyclonal (IgG) to Human CDYL
Human
Western blot, ELISA
HRP Conjugated, Azide-free

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human CDYL
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus
Western blot, ELISA
Unconjugated

Details

Human CDYL
Goat
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1 - 3 µg/ml)
  • ELISA (1:4000)

Specificity and Use

CDYL antibody was raised against synthetic peptide C-SMLKYLQRKIDEF from the C-terminus of human CDYL (NP_004815.3; NP_001137442.1; NP_001137443.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Xenopus (100%); Platypus (92%); Zebrafish (85%).
LS-E25479 - Lyophilized - 100 µg - $145.00
aa586-598
Human CDYL. This antibody is expected to recognize both eported isoforms NP_004815.3; NP_001137442.1; NP_001137443.1). Variants (NP_001137442.1; NP_001137443.1) encode the same isoform.
Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Approx 75 kDa band observed in Human Testes lysates (calculated MW of 66.5 kDa according to NP_004815.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lahn et al , Proc Natl Acad Sci U S A. 2002 Jun 25;99(13):8707-12; PMID: 12072557). Recommended concentration: 1-3 ug/ml. An additional band of unknown identity was also consistently observed at 26 kDa. This band was successfully blocked by incubation with the immunising peptide.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About CDYL

Q9Y232 NM_004824 NP_004815.3

CDYL Antibody, CDY-like Antibody, Chromodomain Y-like protein Antibody, CDY-like, autosomal Antibody, CDYL1 Antibody, Chromodomain protein, Y-like Antibody, DKFZP586C1622 Antibody

Acts as a RE1-silencing transcription factor (REST) corepressor that facilitates histone-lysine N-methyltransferase EHMT2 recruitment and H3K9 dimethylation at REST target genes for repression. Required for chromatin targeting and maximal enzymatic activity of polycomb repressive complex 2 (PRC2); acts as a positive regulator of PRC2 activity by bridging the pre-existing histone H3K27me3 and newly recruited PRC2 on neighboring nucleosomes.

Western blot

Western blot
CDYL antibody staining (1 ug/ml) of Human Testes lysate (RIPA buffer, 35g total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.
 

Requested From: United States
Date Requested: 12/9/2016

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