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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDT1 Antibody (aa400‑449) LS‑C82811
CDT1 antibody LS-C82811 is an unconjugated rabbit polyclonal antibody to CDT1 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C82811-50
50 µg
Unavailable

Popular CDT1 Products

Antibody
Species: Human, Mouse, Rat
Applications: Western blot, Immunoprecipitation
Detection of Human CDT1 by Immunohistochemistry. Sample: FFPE section of human pancreatic islet cell tumor. Antibody: Affinity purified rabbit anti-CDT1 used at a dilution of 1:250.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence
Detection of Human CDT1 by Immunohistochemistry. Sample: FFPE section of human islet cell carcinoma. Antibody: Affinity purified rabbit anti-CDT` used at a dilution of 1:1000 (1 ug/ml).
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Positive WB detected in:HepG2 whole cell lysate,Hela whole cell lysate,Mouse kidney tissue;All lanes:CDT1 antibody at 3?g/ml;Secondary;Goat polyclonal to rabbit IgG at 1/50000 dilution;Predicted band size: 61 KDa;Observed band size: 61 KDa;
Species: Human, Mouse
Applications: Western blot, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA

Product Description

CDT1 antibody LS-C82811 is an unconjugated rabbit polyclonal antibody to CDT1 from human, mouse, rat and other species. Validated for WB.
About CDT1
Cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene. Q9H211 Q9H211

CDT1 Antibody, Double parked homolog Antibody, DUP Antibody, RIS2 Antibody, DNA replication factor Cdt1 Antibody


Specifications

Target
Human CDT1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bat, Hamster, Pig (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bovine, Rabbit (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
CDT1 antibody was raised against synthetic peptide located between aa400-449 of human CDT1 (Q9H211, NP_112190). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Mouse, Rat, Hamster, Bat, Pig (100%); Monkey, Bovine, Rabbit (92%).
Blocking Peptide
LS-E16899 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C82811. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa400-449
Specificity
Human CDT1
Usage
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1.0 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Lane A: Marker. Lane B: HepG2 cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/20/2018

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