LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • EIA Competition Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-CCNL1 / Cyclin L1 Antibody (aa314-369) IHC-plus™ LS-B79

Note: This antibody replaces LS-C19080
See our new CCNL1 / Cyclin L1 ELISA Kits
Mouse/Human/Rat CCNL1 / Cyclin L1 Cell-Based ELISA Kit - LS-F1932
(kits do not necessarily contain the antibody listed on this page)

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

CCNL1 / Cyclin L1 Antibody LS-B79 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-CCNL1 / Cyclin L1 antibody IHC of human thymus.
Western blot
Western blot of Protein A Purified anti-Cyclin L1a antibody shows detection of a band ~59 kDa...

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Human CCNL1 / Cyclin L1
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human CCNL1 / Cyclin L1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Protein A purified

Applications

IHC - Paraffin (10 - 20 µg/ml), Western blot (1:2000 - 1:5000), Immunoprecipitation, ELISA (1:5000 - 1:25000)

Specificity and Use

CCNL1 / Cyclin L1 antibody was raised against synthetic polypeptide corresponding to amino acids 314-369 of Human Cyclin L1a protein.
aa314-369
Human Cyclin L1a
Immunohistochemistry: LS-B79 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B79 was determined to be 10-20 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide
50 µl
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.

Publications (1)

1
Role of CDK/cyclin complexes in transcription and RNA splicing. Loyer P, Trembley JH, Katona R, Kidd VJ, Lahti JM. Cellular signalling. 2005 17:1033-51. [PubMed:15935619]

About CCNL1 / Cyclin L1

Q9UK58 NM_020307 NP_064703.1

CCNL1 Antibody, ANIA6A Antibody, Ania-6a Antibody, BM-001 Antibody, Cyclin L ania-6a Antibody, Cyclin L gamma Antibody, Cyclin L1 Antibody, Cyclin-L Antibody, Cyclin-L1 Antibody, PRO1073 Antibody

Cyclin L (also referred to as CCNL) is encoded by two highly related genes Cyclin L1 and Cyclin L2 (CCNL1 and CCNL2, respectively). Cyclin L has been shown to associate with the PITSLRE kinase and is involved in pre-mRNA processing. Both Cyclin L gene products can be alternatively spliced to produce two isoforms (known as 1 and 2 or alpha and beta). Cyclin L2 is ubiquitous, expressed at much higher levels than Cyclin L1, and thus is likely the major partner for the CDK11p110 protein kinase.

Immunohistochemistry

Immunohistochemistry
Anti-CCNL1 / Cyclin L1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B79 concentration 20 ug/ml.

Western blot

Western blot
Western blot of Protein A Purified anti-Cyclin L1a antibody shows detection of a band ~59 kDa corresponding to a Cyclin L1a (arrowhead) present in mouse brain whole cell lysate. Approximately 35g of lysate was separated by 4-20% SDS-PAGE followed by transfer to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:3,500 for 2h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Requested From: United States
Date Requested: 9/4/2015

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2015 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number