Target
Human CCNB1 / Cyclin B1
Synonyms
CCNB1 | CCNB | Cyclin B1 | G2/mitotic-specific cyclin-B1 | G2/mitotic-specific cyclin B1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein G purified
Immunogen
Recombinant human cyclin B1.
Specificity
Specific for human cyclin B1 (~58kD). Does not cross-react with cyclin A or cyclin D. Species cross-reactivity: Human, weak species cross-reactivity with mouse was observed.
Applications
- Western blot (0.5 - 5 µg/ml)
- Immunoprecipitation
Usage
Suitable for use in Western Blot and Immunoprecipitation. Recommended Dilution. Western Blot: 0.5-5 ug/ml detects cyclin B1 in RIPA lysates from human A431 carcinoma cells. Immunoprecipitation: 2 ug precipitated human cyclin B1 and cdc2 kinase from 500 ug of A431 RIPA lysate.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee