Human CCNB1 / Cyclin B1
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- Western blot (0.5 - 5 µg/ml)
- (applications tested for the base form of this product only)
Recombinant human cyclin B1.
Specific for human cyclin B1 (~58kD). Does not cross-react with cyclin A or cyclin D. Species cross-reactivity: Human, weak species cross-reactivity with mouse was observed.
Suitable for use in Western Blot and Immunoprecipitation. Recommended Dilution. Western Blot: 0.5-5 ug/ml detects cyclin B1 in RIPA lysates from human A431 carcinoma cells. Immunoprecipitation: 2 ug precipitated human cyclin B1 and cdc2 kinase from 500 ug of A431 RIPA lysate.
0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide.
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.
This antibody carries the LSBio 100% Guarantee.