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Anti-BUB1B / BubR1 Antibody (aa1-350, clone 8G1) LS-C2771

Ordering

Wt. Vol. Conc. Price
- 100 µl 0.6 mg/ml $335
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Alternate Forms of this Antibody

LS-C141113 Biotin Conjugated
LS-C141114 DY650 Conjugated
LS-C141115 DY488 Conjugated
LS-C141117 DY550 Conjugated

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 8G1] (IgG1,k) to Human BUB1B / BubR1
Human, Mouse
Immunofluorescence, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human BUB1B / BubR1
Mouse
Human, Mouse (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [8G1]
Protein G purified

Applications

Immunofluorescence (1:100), Western blot (1:2000 - 1:5000) (applications tested for the base form of this product only)

Specificity and Use

BUB1B / BubR1 antibody was raised against a portion of hBUBR1 tagged at the N-terminus with pMAL (residues 1-350 of human BUBR1)
aa1-350
hBUBR1. There should be no cross-reactivity with hBUB1.

Packaging

PBS, 0.1% sodium azide.
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
For research use only.

About BUB1B / BubR1

O60566 NM_001211 NP_001202.4

BUB1B Antibody, Bub1A Antibody, BUBR1 Antibody, HBUBR1 Antibody, Protein SSK1 Antibody, SSK1 Antibody, BUB1beta Antibody, MAD3L Antibody

Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE.

Immunofluorescence

Immunofluorescence
Immunofluorescence of an asynchronous cycling population of human cells (U2OS) with LS-C2771. No signal is detected from interphase cells, whereas cells undergoing mitosis accumulate BubR1 at the kinetochores. Image reveals kinetochores at prometaphase. This image was taken for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested.

Western blot

Western blot
BUBR1 detected in HeLa cell lysate using LS-C2771. This image was taken for the base form of this product. Alternate forms, such as conjugated, azide-free, or ready-to-use, have not been tested.

Requested From: United States
Date Requested: 9/27/2016

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