Target
Human BTK
Synonyms
BTK | AGMX1 | AT | ATK | XLA | PSCTK1 | B-cell progenitor kinase | BPK | Bruton tyrosine kinase | Tyrosine-protein kinase BTK | IMD1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Synthetic phosphopeptide (KLH coupled) corresponding to residues around Ser180 of human Btk. Species Sequence Homology: Mouse - 100%.
Specificity
Recognizes overexpressed levels of human Btk only when phosphorylated at Ser180 (Mr 77kD). This antibody cross-reacts with some other proteins.
Applications
- Western blot (1:1000)
- Immunoprecipitation (1:25)
- ELISA
Usage
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Western Blot: 1:1000. For Western Blot, USBio recommends using immunoprecipitated Btk. Immunoprecipitation: 1:25.
Presentation
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 0.02% Sodium Azide, 50% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee