Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
BMP4 antibody was raised against recombinant mouse BMP4.
Recognizes mouse BMP4. Recognizes human and mouse BMP4 in both reduced and non-reduced conditions. Does not cross-react with human BMP2 or human TGF-b1. Species cross-reactivity: human and rat.
Suitable for use in ELISA, Western Blot, Immunocytochemistry and Immunohistochemistry. Western Blot (Reduced and Non-reduced): Using osteoinductive cells, i. e. BFP and Saos-2. Immunocytochemistry: Using osteoinductive cells, i. e. BFP and Saos-2. Immunohistochemistry (paraffin): 1:2.5 with autoclave/citrate buffer antigen retrieval. Demonstrates the presence of BMP4 in developmental bone formation in human embryo growth plate and the alveolar bone of rat embryo. Positive control: Mouse brain extract.
PBS, pH 7.6, 0.1% Sodium Azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
BMP4 is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site.