PCGF4 antibody LS-C4191 is an unconjugated mouse monoclonal antibody to PCGF4 (BMI1) from human, mouse, rat and other species. Validated for ICC, IP and WB.
Mouse BMI1 / PCGF4
Mouse, Human, Rat, Rabbit
(tested or 100% immunogen sequence identity)
Protein G purified
- Western blot (0.2 - 2 µg/ml)
BMI1 / PCGF4 antibody was raised against recombinant Bmi-1 protein corresponding to aa1-202 of mouse Bmi-1.
Recognizes mouse Bmi-1 (triplet), Mr 40-44kD. Species cross-reactivity: human, rat and rabbit.
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Immunoprecipitation: Reported to immunoprecipitate Bmi-1 from mouse embryo protein extracts. Immunocytochemistry: Reported to immunostain U2OS cells fixed with 2% formaldehyde. Positive control: USOS whole cell lysate, K-562 nuclear extract, 0-4 hour embryos or 12-24 hour embryos.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only.