Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
BMI1, BMI-1, Flvi-2/bmi-1, FLVI2/BMI1, PCGF4, Polycomb group protein Bmi1, Polycomb complex protein BMI-1, Dna-binding protein bmi-1, Polycomb group ring finger 4, RING finger protein 51, RNF51
Mouse, Human, Rat, Rabbit
(tested or 100% immunogen sequence identity)
Protein G purified
Western blot (0.2 - 2 µg/ml)
BMI1 / PCGF4 antibody was raised against recombinant Bmi-1 protein corresponding to aa1-202 of mouse Bmi-1.
Recognizes mouse Bmi-1 (triplet), Mr 40-44kD. Species cross-reactivity: human, rat and rabbit.
Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Immunoprecipitation: Reported to immunoprecipitate Bmi-1 from mouse embryo protein extracts. Immunocytochemistry: Reported to immunostain U2OS cells fixed with 2% formaldehyde. Positive control: USOS whole cell lysate, K-562 nuclear extract, 0-4 hour embryos or 12-24 hour embryos.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.