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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BFSP2 Antibody LS‑C17264
BFSP2 antibody LS-C17264 is an unconjugated guinea pig polyclonal antibody to BFSP2 from human and bovine. Validated for IHC and WB.
Antibody
Catalog
Size
Price
LS-C17264-100
100 µl
$680

Popular BFSP2 Products

Immunohistochemistry Dilution at 1:400 and staining in paraffin-embedded human eye tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
IHC of paraffin-embedded Carcinoma of Human pancreas tissue using anti-BFSP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-BFSP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
IHC of paraffin-embedded Human Kidney tissue using anti-BFSP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot

Product Description

BFSP2 Antibody for IHC, WB/Western LS-C17264

Specifications

Target
Bovine BFSP2
Synonyms
BFSP2, 49 kDa cytoskeletal protein, Beaded filament protein CP49, CP47, CP49, LIFL-L, Phakinin, PHAKOSIN
Host
Guinea pig
Reactivity
Bovine, Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
BFSP2 antibody was raised against phakinin purified from calf lens.
Specificity
Specific detection of phakinin (Mr 47000 polypeptide), intermediate filament protein detected in lens. Cross-reacts with filensin (115kD polypeptide also present in lens). Species cross-reactivity: Human and bovine.
Usage
Suitable for use in Western Blot (Western) and Immunohistochemistry (frozen tissue sections). Western Blot (Western): 1:5000 (ECL). Immunohistochemistry: 1:100; Incubation Time 1 hr at RT.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
About BFSP2
More than 99% of the vertebrate ocular lens is comprised of terminally differentiated lens fiber cells. Two lens-specific intermediate filament-like proteins, the protein product of this gene (phakinin), and filensin, are expressed only after fiber cell differentiation has begun. Both proteins are found in a structurally unique cytoskeletal element that is referred to as the beaded filament (BF). Q13515 NM_003571 NP_003562.1


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Requested From: United States
Date Requested: 10/15/2018

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