Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal to E. coli Bacterial Alkaline Phosphatase
IHC - Frozen, Immunofluorescence, Western blot, ELISA
E. coli Bacterial Alkaline Phosphatase
E. coli (tested or 100% immunogen sequence identity)
Ion exchange chromatography
IHC - Frozen
ELISA (1:3000 - 1:15000)
Specificity and Use
Full length E. coli alkaline phosphatase.
Detects bacterial alkaline phosphatase (BAP), a periplasmic enzyme which catalyzes the release of 5'- and 3'-phosphate groups from DNA, RNA and nucleotides. BAP can also dephosphorylate a number of proteins and alkaloids, and has an optimum pH of 8.0. BAP is encoded by the phoA gene, and forms a homodimer of two identical ~47 kD subunits. It is thought that bacteria use BAP to create free phosphate groups for uptake and use. Other functions include dephosphorylating extracellular organic compounds for import into the cell. Does not react with mammalian forms of alkaline phosphatase.
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.