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Anti-AXIN1 / Axin-1 Antibody (aa898-912) LS-C20823

Ordering

Wt. Vol. Conc. Price
200 µg - - $575
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular AXIN1 / Axin-1 Antibodies

Anti-AXIN1 / Axin-1 Antibody (aa850-862) IHC-plus™ LS-B2741
Goat Polyclonal to Human AXIN1 / Axin-1
Human, Monkey, Mouse, Rat, Bat, Bovine, Hamster, Horse, Zebrafish
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-AXIN1 / Axin-1 Antibody (C-Terminus) IHC-plus™ LS-B6982
Rabbit Polyclonal to Human AXIN1 / Axin-1
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-AXIN1 / Axin-1 Antibody (aa329-359) LS-C319730
Rabbit Polyclonal (IgG) to Human AXIN1 / Axin-1
Human
Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Mouse AXIN1 / Axin-1
Mouse, Rat, Hamster
Western blot
Unconjugated

Details

Mouse AXIN1 / Axin-1
Rabbit
Mouse, Rat, Hamster (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein G purified
Unmodified

Applications

Western blot (1 - 2 µg/ml)

Specificity and Use

AXIN1 / Axin-1 antibody was raised against kLH-conjugated, synthetic peptide corresponding to amino acids 898-912 (SQRKAGGGSAPPCDS) of mouse axin. The immunizing sequence is identical in rat and has 13/15 identical amino acids in human. Percent identity by BLAST analysis: Mouse, Rat, Hamster (100%); Gibbon, Baboon, Monkey (93%); Human, Gorilla, Elephant, Dog, Bovine (87%); Marmoset, Panda, Bat (80%).
aa898-912
Recognizes recombinant mouse Myc-tagged Axin expressed in transfected cell lysate, Mr 130kD. Predicted to cross-react with rat and human based on sequence homology.
Western Blot: 1-2 ug/ml detects Axin in RIPA lysates from Cos-1 cells transfected with full length, mouse axin. Lysates of Cos-1 cells untransfected (lane 1) and expressing transfected Axin (lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Axin (1 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

Packaging

0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About AXIN1 / Axin-1

O15169 AF009674 AAC51624.1

AXIN1 Antibody, Axis inhibitor 1 Antibody, AXIN Antibody, Axin 1 Antibody, Axis inhibition protein 1 Antibody, Fused, mouse, homolog of Antibody, HAxin Antibody, PPP1R49 Antibody, Axin-1 Antibody

Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B.

Requested From: United States
Date Requested: 12/4/2016

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