Mouse AXIN1 / Axin-1
Mouse, Rat, Hamster
(tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- Western blot (1 - 2 µg/ml)
- (applications tested for the base form of this product only)
AXIN1 / Axin-1 antibody was raised against kLH-conjugated, synthetic peptide corresponding to amino acids 898-912 (SQRKAGGGSAPPCDS) of mouse axin. The immunizing sequence is identical in rat and has 13/15 identical amino acids in human. Percent identity by BLAST analysis: Mouse, Rat, Hamster (100%); Gibbon, Baboon, Monkey (93%); Human, Gorilla, Elephant, Dog, Bovine (87%); Marmoset, Panda, Bat (80%).
Recognizes recombinant mouse Myc-tagged Axin expressed in transfected cell lysate, Mr 130kD. Predicted to cross-react with rat and human based on sequence homology.
Western Blot: 1-2 ug/ml detects Axin in RIPA lysates from Cos-1 cells transfected with full length, mouse axin. Lysates of Cos-1 cells untransfected (lane 1) and expressing transfected Axin (lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Axin (1 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.