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Anti-ATE1 Antibody (aa71-120) LS-C111185

Ordering

Wt. Vol. Conc. Price
50 µg - - Unavailable

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100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human ATE1
Human, Monkey, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Human ATE1
Rabbit
Human, Monkey, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Mouse, Rat, Bat (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

ATE1 antibody was raised against synthetic peptide located between aa71-120 of human ATE1 (O95260, NP_008972). Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Galago, Marmoset, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig (100%); Mouse, Rat, Bat, Opossum (92%); Lizard (85%).
LS-E17615 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C111185. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa71-120
Human ATE1
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About ATE1

O95260 AF079098 AAD12366.1

ATE1 Antibody, Arginyltransferase 1 Antibody, R-transferase 1 Antibody

Involved in the post-translational conjugation of arginine to the N-terminal aspartate or glutamate of a protein. This arginylation is required for degradation of the protein via the ubiquitin pathway. Does not arginylate cysteine residues.

Western blot

Western blot
ATE1 antibody LS-C111185 Western blot of COLO205 cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: 
Date Requested: 3/27/2017

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