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Anti-ASL / Argininosuccinate Lyase Antibody (aa101-150) LS-C80446

Catalog	Size	Price
LS-C80446-50	50 µg	Unavailable

Related Products

33 ASL / Argininosuccinate Lyase Antibodies

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Anti-ASL / Argininosuccinate Lyase Antibody (aa13-261) LS-C186321

Antibody:	Rabbit Polyclonal (IgG) to Human ASL / Argininosuccinate Lyase
Reactivity:	Human
Application:	IHC, IHC - Paraffin, ICC, Western blot
Format:	Unconjugated

IHC of paraffin-embedded C2C12 xenograft using ASL antibody at 1:500 dilution.

Anti-ASL / Argininosuccinate Lyase Antibody (clone 4C5-1F2) LS-C196699

Antibody:	Mouse Monoclonal [clone 4C5-1F2] (IgG1,k) to Human ASL / Argininosuccinate Lyase
Reactivity:	Human
Application:	Immunofluorescence, Western blot, ELISA
Format:	Unconjugated

Immunofluorescence of monoclonal antibody to ASL on HeLa cell. [antibody concentration 5 ug/ml]

Anti-ASL / Argininosuccinate Lyase Antibody LS-C334658

Antibody:	Rabbit Polyclonal (IgG) to Human ASL / Argininosuccinate Lyase
Reactivity:	Human, Mouse
Application:	IHC, Immunofluorescence, Western blot
Format:	Unconjugated

Immunofluorescence analysis of HeLa cells.

Anti-ASL / Argininosuccinate Lyase Antibody LS-C482577

Antibody:	Rabbit Polyclonal to Human ASL / Argininosuccinate Lyase
Reactivity:	Human, Mouse
Application:	ICC, Immunofluorescence, Western blot
Format:	Unconjugated

Immunofluorescent analysis of ASL staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Anti-ASL / Argininosuccinate Lyase Antibody (aa86-325) IHC-plus™ LS-B14147

Antibody:	Rabbit Polyclonal (IgG) to Human ASL / Argininosuccinate Lyase
Reactivity:	Human
Application:	IHC, IHC - Paraffin, ICC, Western blot
Format:	Unconjugated

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

Antibody:	Rabbit Polyclonal (IgG) to Human ASL / Argininosuccinate Lyase
Reactivity:	Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Application:	Western blot (applications tested for the base form of this product only)
Format:	Unconjugated

Transfected 293T cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Details

Target:	Human ASL / Argininosuccinate Lyase
Host:	Rabbit
Reactivity:	Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Clonality:	IgG Polyclonal
Conjugations:	Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

Western blot
(applications tested for the base form of this product only)

Specificity and Use

Immunogen:

Synthetic peptide located between aa101-150 of human ASL (P04424, NP_000039). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Chicken, Platypus, Xenopus, Frog, Smelt, Stickleback, Zebrafish, Sea squirt (100%); Bloom alga (92%).

Blocking Peptide:

LS-E15335 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C80446. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

aa101-150

Specificity:

Human ASL

Usage:

ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 0.25 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Packaging

Presentation:	Lyophilized from PBS with 2% sucrose
Reconstitution:	50 µl Distilled Water.
Storage:	Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Restrictions:	For research use only.

About ASL / Argininosuccinate Lyase

Uniprot: P04424 NCBI: NM_000048 NP_000039.2

Synonyms: ASL Antibody, ASAL Antibody, Argininosuccinate lyase Antibody, Argininosuccinase Antibody, Arginosuccinase Antibody

Description: ASL / Argininosuccinate Lyase encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency.

(More About ASL / Argininosuccinate Lyase)

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Western blot

Transfected 293T cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From:
Date Requested: 4/21/2018

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