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ASL / Argininosuccinate Lyase Antibody (aa101‑150) LS‑C80446
Argininosuccinate Lyase antibody LS-C80446 is an unconjugated rabbit polyclonal antibody to Argininosuccinate Lyase (ASL) from human, mouse, rat and other species. Validated for WB.
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Species:
Human
Applications:
IHC, IHC - Paraffin, ICC, Western blot
![Immunofluorescence of monoclonal antibody to ASL on HeLa cell. [antibody concentration 5 ug/ml]](https://lsbio-7d62.kxcdn.com//image2/asl-argininosuccinate-lyase-antibody-clone-4c5-1f2-ls-c196699/143937_374609.jpg)
Species:
Human
Applications:
Immunofluorescence, Western blot, ELISA
Species:
Human, Mouse
Applications:
IHC, Immunofluorescence, Western blot
Species:
Human, Mouse
Applications:
ICC, Immunofluorescence, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, ICC, Western blot
Product Description
Argininosuccinate Lyase antibody LS-C80446 is an unconjugated rabbit polyclonal antibody to Argininosuccinate Lyase (ASL) from human, mouse, rat and other species. Validated for WB.
About ASL / Argininosuccinate Lyase
ASL / Argininosuccinate Lyase encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency.
P04424
NM_000048
NP_000039.2
ASL Antibody, ASAL Antibody, Argininosuccinate lyase Antibody, Argininosuccinase Antibody, Arginosuccinase Antibody
Specifications
Target
Human ASL / Argininosuccinate Lyase
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Guinea pig, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- Western blot
- (applications tested for the base form of this product only)
Immunogen
Synthetic peptide located between aa101-150 of human ASL (P04424, NP_000039). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Chicken, Platypus, Xenopus, Frog, Smelt, Stickleback, Zebrafish, Sea squirt (100%); Bloom alga (92%).
Blocking Peptide
LS-E15335 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C80446. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunizing peptide used to generate LS-C80446. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa101-150
Specificity
Human ASL
Usage
ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 0.25 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Customer Reviews (0)
Images
Western blot
Transfected 293T cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
Transfected 293T cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
Transfected 293T cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
Transfected 293T cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Requested From:
Date Requested: 6/19/2018
Date Requested: 6/19/2018
