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ARHGEF25 / GEFT Antibody (aa506‑555) LS‑C82758 Best Seller
GEFT antibody LS-C82758 is an unconjugated rabbit polyclonal antibody to GEFT (ARHGEF25) from human and rat. Validated for WB.
Catalog
Size
Price
LS-C82758-50
50 µg
Unavailable

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Product Description

GEFT antibody LS-C82758 is an unconjugated rabbit polyclonal antibody to GEFT (ARHGEF25) from human and rat. Validated for WB.
About ARHGEF25 / GEFT
May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. Links specifically G alpha q/11-coupled receptors to RHOA activation. May be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. Involved in skeletal myogenesis. Q86VW2 AA463333 Q86VW2

ARHGEF25 Antibody, GEFT Antibody, RhoA/RAC/CDC42 exchange factor Antibody, p63RhoGEF Antibody, RAC/CDC42 exchange factor Antibody


Specifications

Target
Human ARHGEF25 / GEFT
Host
Rabbit
Reactivity
Human, Rat (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bovine, Pig (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
ARHGEF25 / GEFT antibody was raised against synthetic peptide located between aa506-555 of human ARHGEF25 (Q86VW2, NP_891992). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey (100%); Galago, Marmoset, Bovine, Pig (92%); Mouse, Rat, Dog, Rabbit, Horse, Guinea pig (84%).
Blocking Peptide
LS-E19299 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C82758. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa506-555
Specificity
Human ARHGEF25 / GEFT
Usage
ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
50 µl Distilled Water.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Fetal brain lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/25/2018

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