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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ARF1/2/3/4 Antibody (C‑Terminus) LS‑C54527 Best Seller
Note: This antibody replaces LS-C63756
3/4 antibody LS-C54527 is an unconjugated goat polyclonal antibody to 3/4 (ARF1 / 2) from human, mouse, rat and other species. Validated for Peptide-ELISA and WB.
Catalog
Size
Price
LS-C54527-100
100 µg (0.5 mg/ml)
Unavailable

Product Description

3/4 antibody LS-C54527 is an unconjugated goat polyclonal antibody to 3/4 (ARF1 / 2) from human, mouse, rat and other species. Validated for Peptide-ELISA and WB.

Specifications

Target
Human ARF1/2/3/4
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Sheep, Chicken, Xenopus (tested or 100% immunogen sequence identity)
Predicted
Rabbit, Zebrafish (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Failed Applications
  • IHC - Paraffin
Immunogen
ARF1/2/3/4 antibody was raised against synthetic peptide C-EGLDWLSNQLRNQK from the C-terminus of human ADP Ribosylation Factor (ARF1/ARF2/ARF3/ARF4) (NP_001019397.1; NP_001649.1; NP_001019398.1; NP_001019399.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Panda, Dog, Bovine, Horse, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Tick (100%); Rabbit, Trout, Salmon, Stickleback, Zebrafish (93%).
Blocking Peptide
LS-E26231 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C54527. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human ADP Ribosylation Factor (ARF1/ARF2/ARF3/ARF4). This antibody is expected to recognize all four reported isoforms.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Antibody (1 ug/ml) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (1 ug/ml) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Antibody (1 ug/ml) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (1 ug/ml) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Antibody (1 ug/ml) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (1 ug/ml) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

Antibody (1 ug/ml) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (1 ug/ml) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.


Requested From: 
Date Requested: 6/24/2018

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