Yeast Alpha Tubulin

Rat

Yeast, Avian, E. coli, Mammal (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

Unconjugated

Protein G purified

Unmodified

Yeast tubulin.

C-Terminus

Recognizes the alpha subunit of tubulin, specifically binding to so called Tyr-Tubulin, which is produced by post translational modification of tubulin. The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin. Species cross-reactivity: Reacts with tubulin from yeast, avian and mammalian.

• IHC
• IHC - Frozen
• Western blot
• Immunoprecipitation
• ELISA
• Radioimmunoassay

Immunohistochemistry (frozen). ELISA. Immunoprecipitation. Western Blot. Radioimmunoassay.

PBS, pH 7.2, 0.1% Sodium Azide

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.