Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
- Western blot (1:1000)
- Immunoprecipitation (1:50)
AKT3 antibody was raised against synthetic peptide (KLH-coupled) corresponding to residues of human Akt3.
Detects endogenous levels of total Akt3. Does not cross-react with recombinant Akt1 or Akt2. Species cross-reactivity: Human, Mouse, Rabbit.
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation 1:50.
10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 100 ug/ml BSA, 50% glycerol.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.