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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AKT3 Antibody LS‑C3618
AKT3 antibody LS-C3618 is an unconjugated rabbit polyclonal antibody to AKT3 from human, mouse and rat. Validated for IP and WB.
Antibody
Catalog
Size
Price
LS-C3618-100
100 µl
$530

Popular AKT3 Products

Anti-AKT3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1042 concentration 10 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot
Anti-AKT3 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1319 concentration 10 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Western blot
Anti-AKT3 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3935 concentration 5 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Anti-AKT3 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4825 concentration 10 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
Western blot of Mouse anti-AKT3 antibody. Lane 1: GST Tagged recombinant AKT1. Lane 2: GST Tagged recombinant AKT2. Lane 3: GST Tagged recombinant AKT3. Load: 25 ng per lane. Primary antibody: AKT3 antibody at 1:1000 for overnight at 4C. Secondary antibody: Peroxidase mouse secondary antibody at 1:40000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 78 kDa for AKT3. Other band(s): none.
Species: Human
Applications: Western blot, ELISA

Product Description

AKT3 antibody LS-C3618 is an unconjugated rabbit polyclonal antibody to AKT3 from human, mouse and rat. Validated for IP and WB.
About AKT3
AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. Q9Y243 NM_005465 NP_005456.1

AKT3 Antibody, AKT3/PKBgamma Antibody, PRKBG Antibody, PKB gamma Antibody, Protein kinase Akt-3 Antibody, RAC-gamma Antibody, MPPH Antibody, PKB-GAMMA Antibody, PKBG Antibody, Protein kinase B gamma Antibody, RAC-PK-gamma Antibody, STK-2 Antibody


Specifications

Target
Human AKT3
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • Western blot (1:1000)
  • Immunoprecipitation (1:50)
Immunogen
AKT3 antibody was raised against synthetic peptide (KLH-coupled) corresponding to residues of human Akt3.
Specificity
Detects endogenous levels of total Akt3. Does not cross-react with recombinant Akt1 or Akt2. Species cross-reactivity: Human, Mouse, Rabbit.
Usage
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation 1:50.
Presentation
10 mM HEPES-Sodium, pH 7.5, 150 mM NaCl, 100 ug/ml BSA, 50% Glycerol
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Requested From: United States
Date Requested: 9/20/2018

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