Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 25F6.F6.D8] (IgG1) to Human AKT3
Western blot, ELISA
Rabbit Polyclonal (IgG) to Human AKT3
Human, Mouse, Rat
Western blot, Immunoprecipitation
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Western blot (1:1000)
Specificity and Use
AKT3 antibody was raised against synthetic peptide (KLH-coupled) corresponding to residues of human Akt3.
Detects endogenous levels of total Akt3. Does not cross-react with recombinant Akt1 or Akt2. Species cross-reactivity: Human, Mouse, Rabbit.
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation 1:50.
10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 100 ug/ml BSA, 50% glycerol.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
AKT3 Antibody, AKT3/PKBgamma Antibody, PRKBG Antibody, PKB gamma Antibody, Protein kinase Akt-3 Antibody, RAC-gamma Antibody, MPPH Antibody, PKB-GAMMA Antibody, PKBG Antibody, Protein kinase B gamma Antibody, RAC-PK-gamma Antibody, STK-2 Antibody
AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform.