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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AKT1 + AKT2 + AKT3 Antibody (C‑Terminus) LS‑C20398
AKT1 + AKT2 + AKT3 antibody LS-C20398 is an unconjugated rabbit polyclonal antibody to AKT1 + AKT2 + AKT3 from human and mouse. Validated for WB.
Catalog
Size
Price
LS-C20398-200
200 µl
$605
Description
AKT1 + AKT2 + AKT3 antibody LS-C20398 is an unconjugated rabbit polyclonal antibody to AKT1 + AKT2 + AKT3 from human and mouse. Validated for WB.
Target
Human AKT1 + AKT2 + AKT3
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Rat (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
Synthetic phosphopeptide derived from the carboxy-terminus region of human Akt1.
Epitope
C-Terminus
Specificity
Human and mouse Akt1. Rat Akt1 (94% homologous), human Akt2 (65% homologous), and human Akt3 (82% homologous) have not been tested, but are expected to react.
Usage
The antibody has been used for Western blotting applications.
Presentation
1 mg/ml BSA (IgG, Protease free), PBS (without Mg2+, Ca2+), pH 7.2, 0.1% Sodium Azide, 50% Glycerol
Restrictions
For research use only.

Popular AKT1 + AKT2 + AKT3 Products

Anti-AKT1/2/3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Species: Human, Mouse, Rat, Chicken
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Akt (Phospho-Ser473) Antibody. The picture on the right is blocked with the phospho peptide.
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Immunohistochemical analysis of AKT staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. w
Species: Human, Mouse, Rat, Bovine, Sheep
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Immunohistochemical analysis of AKT (pS246) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat, Bovine, Chicken, Zebrafish
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Immunohistochemical analysis of AKT staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat, Bovine, Dog, Chicken, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot

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Requested From: United States
Date Requested: 12/16/2018
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