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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AKR1D1 Antibody (aa113‑162) LS‑C135886
AKR1D1 antibody LS-C135886 is an unconjugated rabbit polyclonal antibody to AKR1D1 from human, rat, bovine and other species. Validated for WB.
Catalog
Size
Price
LS-C135886-50
50 µg
Unavailable

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Antibody
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Applications: IHC, Western blot, ELISA

Product Description

AKR1D1 antibody LS-C135886 is an unconjugated rabbit polyclonal antibody to AKR1D1 from human, rat, bovine and other species. Validated for WB.
About AKR1D1
Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4-cholesten-3-one can also act as substrates. P51857 NM_005989 NP_005980.1

AKR1D1 Antibody, 3o5bred Antibody, SRD5B1 Antibody, CBAS2 Antibody


Specifications

Target
Human AKR1D1
Host
Rabbit
Reactivity
Human, Rat, Bovine, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Dog, Horse, Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
AKR1D1 antibody was raised against synthetic peptide located between aa113-162 of human AKR1D1 (P51857, NP_005980). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Rabbit (100%); Gorilla, Monkey, Marmoset, Mouse, Dog, Horse, Xenopus (92%); Galago, Elephant, Bovine, Pig, Chicken, Smelt, Sablefish (85%); Turkey (84%); Rat (76%).
Blocking Peptide
LS-E22252 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C135886. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa113-162
Specificity
Human AKR1D1
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

AKR1D1 antibody LS-C135886 Western blot of 8226 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
AKR1D1 antibody LS-C135886 Western blot of 8226 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

AKR1D1 antibody LS-C135886 Western blot of 8226 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
AKR1D1 antibody LS-C135886 Western blot of 8226 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

AKR1D1 antibody LS-C135886 Western blot of 8226 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
AKR1D1 antibody LS-C135886 Western blot of 8226 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

AKR1D1 antibody LS-C135886 Western blot of 8226 Cell lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
AKR1D1 antibody LS-C135886 Western blot of 8226 Cell lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/21/2018

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