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Anti-AICDA / AID Antibody (C-Terminus) IHC-plus™ LS-B16

Note: This antibody replaces LS-C422, LS-C56869, LS-C57132, LS-C121339, LS-C43159

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular AICDA / AID Antibodies

Anti-AICDA / AID Antibody (C-Terminus) IHC-plus™ LS-B16
Rabbit Polyclonal to Human AICDA / AID
Human, Mouse
IHC - Paraffin, ICC, Western blot
Unconjugated
Immunohistochemistry Image
Anti-AICDA / AID Antibody (N-Terminus) IHC-plus™ LS-B6738
Rabbit Polyclonal to Human AICDA / AID
Human
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-AICDA / AID Antibody IHC-plus™ LS-B13191
Rabbit Polyclonal to Human AICDA / AID
Human, Mouse, Rat, Dog, Guinea pig, Horse, Pig, Rabbit, Cow
IHC - Paraffin
Unconjugated
Immunohistochemistry - Paraffin Image

100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal to Human AICDA / AID
Human, Mouse
IHC - Paraffin, ICC, Western blot
Unconjugated

Details

Human AICDA / AID
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • ICC (10 µg/ml)
  • Western blot (1 - 2 µg/ml)

Specificity and Use

AICDA / AID antibody was raised against synthetic peptide from human AICDA.
LS-E30009 - Liquid - 50 µg - $145.00
C-Terminus
peptide corresponding to 14 amino acids near the carboxy-terminus of human AID
Immunohistochemistry: LS-B16 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B16 was determined to be 5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

Publications (1)

1
Primary central nervous system lymphoma: tumor-related clones exist in the blood and bone marrow with evidence for separate development. McCann KJ, Ashton-Key M, Smith K, Stevenson FK, Ottensmeier CH. Blood. 2009 113:4677-80. (IHC; Human) [PubMed:19096008]

About AICDA / AID

Q9GZX7 NM_020661 NP_065712.1

AICDA Antibody, AID Antibody, ARP2 Antibody, CDA2 Antibody, Cytidine aminohydrolase Antibody, HIGM2 Antibody

Activation-induced cytidine deaminase (AICDA) was initially discovered as a homolog of the apolipoprotein B RNA-editing cytidine deaminase 1 (APOBEC1) that showed cytidine deaminase properties in stimulated B cell lines. AICDA is necessary for somatic hypermutation and class switch recombination in B cells, but inappropriate or dysregulated expression AID is often found in tumors and B cell neoplasms.

Immunohistochemistry

Immunohistochemistry
Anti-AICDA antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B16 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-AICDA antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B16 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry
Immunocytochemistry of AID in Ramos cells with Ramos antibody at 10 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of AID in Ramos cells with AID antibody at 20 ug/ml.

Western blot

Western blot
Western blot of AID in Ramos whole cell lysate with AID antibody at 2 ug/ml in either the (A) absence or (B) presence of blocking peptide.
 

Requested From: United States
Date Requested: 12/5/2016

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