- IHC - Paraffin
- Western blot
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Agmatine antibody was raised against agmatine (BSA coupled).
Recognizes Agmatine. Does not cross-react with arginine, glutamate or other amino acids.
Suitable for use in Immunohistochemistry, Immunocytochemistry and Western Blot. Immunohistochemistry: 1:100 for 0.1-2.5% glutaraldehyde fixed tissues. Optimal fixation: 0.1-2.5% glutaraldehyde, 1% formalin. Minimum glutaraldehyde: 0.1%; works on paraffin. embedded tissue (fixed with glutaraldehyde) but preferably epoxy embedded specifically developed for post-embedding protocols. Living, isolated goldfish retinas were maintained for. 10 minutes in an oxygenated physiological solution that preserves normal neuronal activity under constant lighting. The medium also contained 10 mM agmatine. After the incubation period retinas were fixed in a conventional 2.5% glutaraldehyde buffer and processed for immunohistochemistry using 250nm sections probed with a gold-labeled secondary antibody. The treated retinas were exposed to 125 M kainate during the incubation. Kainate opens both AMPA and kainate selective ionotropic channels through which agmatine can enter cells. Immunocytochemistry: On cells with glutamate-gated ion channels.
PBS, 1% goat serum, 0.05% Thimerosal.
For research use only.
This antibody carries the LSBio 100% Guarantee.