Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
Recognizes the human CD97 cell surface antigen, a 74-89kD glycoprotein expressed by granulocytes, monocytes, some activated lymphocytes and weakly by resting lymphocytes (5-15%). This antibody has been reported to detect CD97 antigen weakly in Western blotting.
Western Blot: Reported. Flow Cytometry: 1:200-1:400. Use 10 ul of the suggested working dilution to label 1E6 cells or 100 ul whole blood. Immunoprecipitation.
PBS, pH 7.2, 0.1% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
ADGRE5 / CD97 is a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex.