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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ADGRE5 / CD97 Antibody LS‑C5747
CD97 antibody LS-C5747 is an unconjugated mouse monoclonal antibody to human CD97 (ADGRE5). Validated for Flow, IP and WB.
Catalog
Size
Price
LS-C5747-200
200 µg
$755
Description
CD97 antibody LS-C5747 is an unconjugated mouse monoclonal antibody to human CD97 (ADGRE5). Validated for Flow, IP and WB.
Target
Human ADGRE5 / CD97
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, Biotin, FITC, HRP, PE.
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot
  • Immunoprecipitation
  • Flow Cytometry (1:200 - 1:400)
  • (applications tested for the base form of this product only)
Specificity
Recognizes the human CD97 cell surface antigen, a 74-89kD glycoprotein expressed by granulocytes, monocytes, some activated lymphocytes and weakly by resting lymphocytes (5-15%). This antibody has been reported to detect CD97 antigen weakly in Western blotting.
Usage
Western Blot: Reported. Flow Cytometry: 1:200-1:400. Use 10 ul of the suggested working dilution to label 1E6 cells or 100 ul whole blood. Immunoprecipitation.
Presentation
PBS, pH 7.2, 0.1% Sodium Azide
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About ADGRE5 / CD97
P48960 NM_078481 NP_510966.1

Popular ADGRE5 / CD97 Products

Anti-CD97 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Western blot
Anti-CD97 Antibody - Western Blot. Western blot of Protein A purified anti-CD97 antibody shows detection of bands corresponding to free Fc-CD97- (5EGF) (lower arrowhead) and Fc-CD97- (5EGF) present as a complex (upper arrowhead) in lysates from COS cells. The left lane contains lysate from cells transfected with control DNA. The right lane contains lysate from COS cells expressing Fc-CD97- (5EGF). No staining was noted from bone marrow lysates taken from CD97 knockout mice. The identity of the band at ~65 kD appearing in all lanes is not known. The formation of the CD97 complex is currently under investigation. Approximately 10 ul of lysate was used in each lane. A 1:1000 dilution of the primary antibody was used. The image was processed using a 10-sec exposure. Personal Communication. Yvona Ward. NIH, NCI, CCR, Bethesda, MD.
Species: Mouse, Human
Applications: Immunofluorescence, Western blot
Immunohistochemistry Dilution at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, ELISA
Anti-CD97 antibody IHC of human bone marrow. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin, ICC
Antibody
Species: Human
Applications: ICC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA

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Requested From: United States
Date Requested: 12/18/2018
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