FYB antibody LS-C3555 is an unconjugated rabbit polyclonal antibody to FYB (ADAP) from human, mouse, rat and other species. Validated for IP and WB.
Mouse, Human, Monkey, Rat, Bat, Bovine, Dog, Horse, Pig
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- Western blot (0.5 - 2 µg/ml)
- (applications tested for the base form of this product only)
ADAP / FYB antibody was raised against synthetic peptide corresponding to amino acids 755-768 (DNDGEIYDDIADGC) of mouse Fyn binding protein (ADAP/SLAP-130/Fyb) (KLH). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Bovine, Dog, Bat, Horse, Pig (100%); Platypus (93%).
Recognizes ADAP/SLAP-130/Fyb, Mr ~130kD. An unknown protein was also detected, Mr ~40kD that is also inhibited by the immunizing peptide. Species sequence homology: Human and rat.
Suitable for use in Western Blot and Immunoprecipitation. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Immunoprecipitation: 4 ug immunoprecipitated ADAP/SLAP-130/Fyb from 500 ug of RAW. 264.7 macrophage RIPA lysate.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.