(tested or 100% immunogen sequence identity)
Rat (at least 90% immunogen sequence identity)
- Western blot (1:500 - 1:2000)
ADAM12 antibody was raised against 13 amino acid synthetic peptide from the C-terminus of the mouse ADAM-12 protein (HQVPRPSHNAYIK). Percent identity by BLAST analysis: Mouse (100%); Rat (92%); Hamster, Horse (85%).
Recognizes mouse ADAM 12. Cross-reactivity with other ADAM family members untested.
Suitable for use in Western Blot. Not recommended for Flow Cytometry. Western Blot: 1:500-1:2000; 5% nonfat milk in PBS for blocking. Do not dilute the primary antibody in milk; Simple TBS or PBS is recommended. Variable size on gel expected, as there are several processed forms of the protein.
No preservatives added
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.