LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
ACTN1 Antibody (N‑Terminus) LS‑C102772
ACTN1 antibody LS-C102772 is an unconjugated rabbit polyclonal antibody to ACTN1 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C102772-50
50 µg
Unavailable

Popular ACTN1 Products

Antibody
Species: Human
Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation
IHC of paraffin-embedded Human Kidney tissue using anti-ACTN1 mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Antibody
Species: Human, Monkey, Mouse, Rat, Hamster
Applications: ICC, Immunofluorescence, Western blot
Antibody
Species: Human, Mouse, Rat, Rabbit
Applications: IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence, Western blot, Immunoprecipitation
Immunohistochemistry of paraffin-embedded human kidney using ACTN1 antibody at dilution of 1:200 (200x lens).
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot

Product Description

ACTN1 antibody LS-C102772 is an unconjugated rabbit polyclonal antibody to ACTN1 from human, mouse, rat and other species. Validated for WB.
About ACTN1
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. P12814 NM_001102 NP_001093.1

ACTN1 Antibody, Alpha-actinin-1 Antibody, Actinin 1 smooth muscle Antibody, Actinin, alpha 1 Antibody, F-actin cross-linking protein Antibody, Non-muscle alpha-actinin-1 Antibody, ASMA Antibody


Specifications

Target
Human ACTN1
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Rabbit, Chicken, Zebrafish (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Failed Applications
  • IHC - Paraffin
Immunogen
ACTN1 antibody was raised against synthetic peptide from N-Terminus of human ACTN1 (P12814, NP_001093). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Opossum, Guinea pig, Chicken (100%); Zebrafish (84%); Xenopus (83%).
Blocking Peptide
LS-E21361 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C102772. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
N-Terminus
Specificity
Human ACTN1 / ASMA
Usage
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

Publications (0)


Customer Reviews (0)


Images


Western blot

ACTN1 / ASMA antibody LS-C102772 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ACTN1 / ASMA antibody LS-C102772 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ACTN1 / ASMA antibody LS-C102772 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ACTN1 / ASMA antibody LS-C102772 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ACTN1 / ASMA antibody LS-C102772 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ACTN1 / ASMA antibody LS-C102772 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

ACTN1 / ASMA antibody LS-C102772 Western blot of 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ACTN1 / ASMA antibody LS-C102772 Western blot of 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/24/2018

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number