Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(tested or 100% immunogen sequence identity)
Tissue culture supernatant
IHC - Frozen
ICC (1:20 - 1:50)
Western blot (1:20 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
ABCC3 / MRP3 antibody was raised against recombinant human MRP3 (multidrug resistance-associated protein 3) (aa 830-949).
Recognizes an internal epitope of human MRP3. Does not cross-react with human P-glycoprotein, MDR3 P-glycoprotein, MRP1, MRP2 (cMOAT) or MRP5. Does not cross-react with mouse, rat or guinea pig MRP2.
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Flow Cytometry: Weak, cell permeabilization required. Western Blot: Use 1:20-1:50 and IgG (HRP) anti-mouse. Immunocytochemistry: 1:20-1:50 on acetone or formaldehyde fixed cytospin preparations. Immunohistochemistry (Frozen sections): 1:20 on acetone fixed frozen sections followed by incubation with IgG rabbit anti-mouse and a monoclonal mouse APAAP complex. Alternatively, after incubation with the primary antibody (1:20) and washing, slides can be incubated with IgG (Biotin) rabbit anti-mouse and Streptavidin (HRP).
ABCC3 / MRP3 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance.
Transporter function and cyclic AMP turnover in normal colonic mucosa from patients with and without colorectal neoplasia. Kleberg K, Jensen GM, Christensen DP, Lundh M, Grunnet LG, Knuhtsen S, Poulsen SS, Hansen MB, Bindslev N. BMC gastroenterology. 2012 12:78. (IHC; Human)[Full Text Article]