MRP3 antibody LS-C16477 is an unconjugated mouse monoclonal antibody to human MRP3 (ABCC3). Validated for ELISA, Flow, ICC, IF, IHC and WB. Cited in 1 publication.
Human ABCC3 / MRP3
(tested or 100% immunogen sequence identity)
Tissue culture supernatant
- IHC - Frozen
- ICC (1:20 - 1:50)
- Western blot (1:20 - 1:50)
- Flow Cytometry
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ABCC3 / MRP3 antibody was raised against recombinant human MRP3 (multidrug resistance-associated protein 3) (aa 830-949).
Recognizes an internal epitope of human MRP3. Does not cross-react with human P-glycoprotein, MDR3 P-glycoprotein, MRP1, MRP2 (cMOAT) or MRP5. Does not cross-react with mouse, rat or guinea pig MRP2.
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Flow Cytometry: Weak, cell permeabilization required. Western Blot: Use 1:20-1:50 and IgG (HRP) anti-mouse. Immunocytochemistry: 1:20-1:50 on acetone or formaldehyde fixed cytospin preparations. Immunohistochemistry (Frozen sections): 1:20 on acetone fixed frozen sections followed by incubation with IgG rabbit anti-mouse and a monoclonal mouse APAAP complex. Alternatively, after incubation with the primary antibody (1:20) and washing, slides can be incubated with IgG (Biotin) rabbit anti-mouse and Streptavidin (HRP).
Tissue Culture Supernatant, 0.1% Sodium Azide, 0.7% BSA
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.