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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
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Catalog Number Size Price
LS-B6644-50 50 µl $485 
14-3-3 Antibody - Anti-14-3-3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

IHCPlus™ 14‑3‑3 Antibody Polyclonal Serum IHC,IP,WB LS‑B6644

IHCPlus™ 14‑3‑3 Antibody Polyclonal Serum IHC,IP,WB LS‑B6644

Note: This antibody replaces LS-C42378
Antibody:
Rabbit Polyclonal to Sheep 14-3-3
Application:
IHC, IHC-P, WB, IP
Reactivity:
Sheep
Format:
Unconjugated, Serum
Price
Catalog Number
$485 
LS-B6644-50
50 µl
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Sheep 14-3-3
Application:
IHC, IHC-P, WB, IP
Reactivity:
Sheep
Format:
Unconjugated, Serum

Specifications

Description
14-3-3 antibody LS-B6644 is an unconjugated rabbit polyclonal antibody to sheep 14-3-3. Validated for IHC, IP and WB.
Target
Sheep 14-3-3
Host
Rabbit
Reactivity
Sheep (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Serum
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • Western blot (1:2000)
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A mixture of synthetic peptides corresponding to all isoforms of sheep 14-3-3
Specificity
Recognizes all isoforms of the 14-3-3 protein family. The 14-3-3 family consists of 30 kD proteins involved in multiple cellular processes including protein kinase signalling pathways, regulation of cell cycle progression, transcription and intracellular trafficking. Protein interactions with 14-3-3 show distinct preferences for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.
Usage
Western Blot: Detects a band of approximately 30kD in HEK293 cell lysates. Immunohistology: This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Presentation
0.09% Sodium Azide
Storage
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Validation

14-3-3 Antibody - Anti-14-3-3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-14-3-3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-14-3-3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

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Requested From: United States
Date Requested: 12/10/2019