Detects ~29-30 kD proteins, corresponding to the apparent molecular mass of the beta and zeta isoforms of 14-3-3 on SDS-PAGE immunoblots, in samples from human, mouse, rat, monkey, rabbit, dog, sheep, pig and bovine origins. This antibody is suitable as a Golgi marker as the epitopes recognized are on the cytoplasmic face of the Golgi membrane. It also reacts specifically with two members of the 14-3-3 protein family: the acidic human IEF58 (~29 kD) and IEF57a (~30 kD), which correspond to 14-3-3 beta and zeta, respectively.
PBS, pH 7.2, 0.1 mM PMSF, 0.09% Sodium Azide, 50% Glycerol
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
This antibody carries the LSBio 100% Guarantee.