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| Catalog Number | Size | Price |
|---|---|---|
| LS-B86-0.1 | 0.1 ml (1 mg/ml) | $460 |
IHC‑plus™ Polyclonal Rabbit anti‑Human DEFB3 / Beta Defensin 3 Antibody (IHC) LS‑B86
IHC‑plus™ Polyclonal Rabbit anti‑Human DEFB3 / Beta Defensin 3 Antibody (IHC) LS‑B86
Note: This antibody replaces LS-C2782, LS-C35131
Antibody:
DEFB3 / Beta Defensin 3 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IHC-Fr
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
DEFB3 / Beta Defensin 3 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IHC-Fr
Reactivity:
Human, Rat
Format:
Unconjugated, Unmodified
Specifications
Description
Beta Defensin 3 antibody LS-B86 is an unconjugated rabbit polyclonal antibody to Beta Defensin 3 (DEFB3) from human. It is reactive with human and rat. Validated for IHC. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
Host
Rabbit
Reactivity
Human, Rat
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide: SCLPKEEQIGK corresponding to amino acids 44-54 of Human beta 3 Defensin
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- IHC - Frozen (1:10 - 1:500)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B86 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B86 was determined to be 10 ug/ml.
Presentation
PBS, 0.02% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Validation
Anti-Beta Defensin 3 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-Beta Defensin 3 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ DEFB3 / Beta Defensin 3 Antibody for IHC LS-B86 has an LSBio Rating of
Publications (4.2)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (3)
Unique profile of antimicrobial peptide expression in polymorphic light eruption lesions compared to healthy skin, atopic dermatitis, and psoriasis. Patra V, Mayer G, Gruber-Wackernagel A, Horn M, Lembo S, Wolf P. Photodermatology, photoimmunology & photomedicine. 2018 March;34:137-144. (Human)
Overexpressions of hBD-2, hBD-3, and hCAP18/LL-37 in Gingiva of Diabetics with Periodontitis. Dogukan Yilmaz, Guliz N. Güncü, Eija Könönen, Emre Baris, Feriha Çaglayan, Ulvi Kahraman Gursoy. Immunobiology. 2015 1219-1226.
Regulation of hBD-2, hBD-3, hCAP18/LL37, and Proinflammatory Cytokine Secretion by Human Milk Oligosaccharides in an Organotypic Oral Mucosal Model. Ulvi K Gürsoy, Krista Salli, Eva Söderling, Mervi Gürsoy, Johanna Hirvonen, Arthur C Ouwehand. Pathogens. 2021 Jun;10:739.
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Featured Products
Species:
Human, Monkey
Applications:
ELISA, Radioimmunoassay
Species:
Human
Applications:
Western blot, ELISA
Reactivity:
Mouse
Range:
0.156-10 ng/ml
Reactivity:
Mouse
Range:
0.156-10 ng/ml
Reactivity:
Human
Range:
0.41-100 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
