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PNP / Nucleoside Phosphorylase

purine nucleoside phosphorylase

Nucleoside Phosphorylase / PNP is an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2.

Gene Name: purine nucleoside phosphorylase
Synonyms: PNP, Inosine phosphorylase, NP, Nucleoside phosphorylase, PUNP, PRO1837
Target Sequences: NM_000270 NP_000261.2 P00491

Publications (1)

1
Partial Purine Nucleoside Phosphorylase Deficiency Helps Determine Minimal Activity Required for Immune and Neurological Development. Eyal Grunebaum, Nicholas Campbell, Matilde Leon-Ponte, Xiaobai Xu, Hugo Chapdelaine. Frontiers in immunology. 2020 July;11:1257. [Full Text Article] [PubMed:32695102] [PMC:PMC7338719] Related Antibodies: LS-C137543.

☰ Filters
Antibodies (65)
Proteins (10)
ELISA Kits (1)
Custom (1)
Over-Expression Lysate (2)
Primary (65)
Recombinant (8)
PNP / Nucleoside Phosphorylase (76)
Human (53)
Mouse (14)
Rat (11)
Bovine (6)
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Chimpanzee (3)
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Micrococcus luteus (2)
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IHC (12)
IHC-P (6)
WB (59)
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ELISA (18)
ICC (1)
ID (2)
IE (2)
IF (5)
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rabbit (60)
mouse (2)
goat (1)
human (1)
sheep (1)
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monoclonal mc (2)
polyclonal pc (63)
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aa1-289 (7)
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aa145-178 (1)
aa239-289 (1)
aa68-289 (1)
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Purified (4)
PNP / Nucleoside Phosphorylase Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
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PNP / Nucleoside Phosphorylase Mouse anti-Human Monoclonal (aa68-289) Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated
100 µl/$445
PNP / Nucleoside Phosphorylase Antibody - Anti-Nucleoside Phosphorylase / PNP antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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PNP / Nucleoside Phosphorylase Goat anti-Human Polyclonal (aa142-153) Antibody
Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50 µg/$485
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PNP / Nucleoside Phosphorylase Rabbit anti-Bovine Polyclonal Antibody
Bovine
ELISA, IF, IHC, WB
Unconjugated
10 mg/$335
Select
PNP / Nucleoside Phosphorylase Rabbit anti-Micrococcus luteus Polyclonal Antibody
Micrococcus luteus
ELISA, ID, IE, WB
Unconjugated
1 mg/$890
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PNP / Nucleoside Phosphorylase Rabbit anti-Micrococcus luteus Polyclonal Antibody
Micrococcus luteus
ELISA, ID, IE, WB
Unconjugated
5 mg/$558
PNP / Nucleoside Phosphorylase Antibody - IHC analysis of PNP using anti-PNP antibody. PNP was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-PNP Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
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PNP / Nucleoside Phosphorylase Rabbit anti-Human Polyclonal Antibody
Human
Flo, ICC, IHC, WB
Unconjugated
10 µg/$318; 100 µg/$470
PNP / Nucleoside Phosphorylase Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 5 ug/ml.
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PNP / Nucleoside Phosphorylase Rabbit anti-Human Polyclonal (aa239-289) Antibody
Human
IHC, IHC-P, IP, WB
Unconjugated
50 µl/$460
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PNP / Nucleoside Phosphorylase Rabbit anti-Bovine Polyclonal Antibody
Bovine
ELISA, IF, IHC, WB
Unconjugated
1 mg/$520
PNP / Nucleoside Phosphorylase Antibody - Immunohistochemistry of paraffin-embedded human brain tissue using antibody at 1:100 dilution.
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PNP / Nucleoside Phosphorylase Rabbit anti-Human Polyclonal (aa1-289) Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µl/$294; 100 µl/$360
Select
PNP / Nucleoside Phosphorylase Rabbit anti-Bovine Polyclonal (Biotin) Antibody
Bovine
ELISA, IF, IHC, WB
Biotin Conjugated
1 ml/$438
PNP / Nucleoside Phosphorylase Antibody - IHC testing of FFPE human renal cancer tissue with Purine nucleoside phosphorylase antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
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PNP / Nucleoside Phosphorylase Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
Flo, IHC-P, WB
Unconjugated
100 µg/$575
PNP / Nucleoside Phosphorylase Antibody - 1:100 staining human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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PNP / Nucleoside Phosphorylase Rabbit anti-Human Polyclonal Antibody
Human
IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
Select
PNP / Nucleoside Phosphorylase Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
50 µl/$356; 100 µl/$461
Select
PNP / Nucleoside Phosphorylase Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IP, WB
Unconjugated
100 µl/$412
PNP / Nucleoside Phosphorylase Antibody - Western blot analysis of extracts of various cell lines.
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PNP / Nucleoside Phosphorylase Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
PNP / Nucleoside Phosphorylase Antibody - Immunofluorescence analysis of U2OS cells using PNP antibody. Blue: DAPI for nuclear staining.
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PNP / Nucleoside Phosphorylase Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
PNP / Nucleoside Phosphorylase Antibody - Anti-PNP rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 32 kDa.
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PNP / Nucleoside Phosphorylase Rabbit anti-Human Polyclonal (Preservative Free) Antibody
Human
ELISA, IP, WB
Unconjugated, Preservative Free
100 µl/$412
PNP / Nucleoside Phosphorylase Antibody - Western Blot analysis of NP expression in transfected 293T cell line by NP monoclonal antibody (M01), clone 6E5.Lane 1: NP transfected lysate (Predicted MW: 32.1 KDa).Lane 2: Non-transfected lysate.
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PNP / Nucleoside Phosphorylase Mouse anti-Human Monoclonal (6E5) Antibody
Human
ELISA, WB
Unconjugated
100 µg/$423
Select
PNP / Nucleoside Phosphorylase Rabbit anti-Human Polyclonal Antibody
Human
IHC, IHC-P
Unconjugated
100 µl/$412
Select
PNP / Nucleoside Phosphorylase Rabbit anti-Human Polyclonal Antibody
Human
ELISA, WB
Unconjugated
100 µg/$331
PNP / Nucleoside Phosphorylase Antibody - Western blot All lanes: Purine nucleoside phosphorylase antibody at 16µg/ml Lane 1: Jurkat whole cell lysate Lane 2: K562 whole cell lysate Lane 3: U937 whole cell lysate Lane 4: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 32 kDa Observed band size: 32 kDa
Select
PNP / Nucleoside Phosphorylase Rabbit anti-Human Polyclonal (aa1-289) Antibody
Human
ELISA, WB
Unconjugated
50 µg/$294; 100 µg/$360
Select
PNP / Nucleoside Phosphorylase Rabbit anti-Human Polyclonal (aa1-289) (HRP) Antibody
Human
ELISA, WB
HRP Conjugated
50 µg/$294; 100 µg/$360
Select
PNP / Nucleoside Phosphorylase Rabbit anti-Human Polyclonal (aa1-289) (Biotin) Antibody
Human
ELISA, WB
Biotin Conjugated
50 µg/$294; 100 µg/$360
Select
PNP / Nucleoside Phosphorylase Rabbit anti-Human Polyclonal (aa1-289) (FITC) Antibody
Human
ELISA, WB
FITC Conjugated
50 µg/$294; 100 µg/$360
PNP / Nucleoside Phosphorylase Antibody - Western blot. All lanes: PNP antibody at 6 ug/ml. Lane 1: Jurkat whole cell lysate. Lane 2: k562 whole cell lysate. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 32 kDa. Observed band size: 32 kDa.
Select
PNP / Nucleoside Phosphorylase Rabbit anti-Human Polyclonal (aa1-289) Antibody
Human
ELISA, WB
Unconjugated
50 µl/$294; 100 µl/$360
Viewing 1-25 of 76 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).