Binding
Measured by its binding ability in a functional ELISA. Immobilized human ErbB2 at 2.5 µg/ml can bind Herceptin with a linear range of 3.2-80 ng/ml.
Proliferation
Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.5-3 µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Measured by its binding ability in a functional ELISA. Immobilized human ErbB2 at 2.5 µg/ml can bind Herceptin with a linear range of 3.2-80 ng/ml.
Proliferation
Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.5-3 µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Measured by its binding ability in a functional ELISA. Immobilized human ErbB2 at 2.5 µg/ml can bind Herceptin with a linear range of 3.2-80 ng/ml.
Proliferation
Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.5-3 µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Measured by its binding ability in a functional ELISA. Immobilized human ErbB2 at 2.5 µg/ml can bind Herceptin with a linear range of 3.2-80 ng/ml.
Proliferation
Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.5-3 µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis