Products
Research Areas
COVID-19
Resources
Login
Quick Order
Cart Cart lightblue
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us

Locations


Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464


Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers



Tel: +1 (800) 227-6666


Contact Us



Additional Contact Details

Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-G47281-100 100 µg $441 
LS-G47281-1 1 mg $1,991 
ERBB2 / HER2 Protein - Measured by its binding ability in a functional ELISA. Immobilized human ErbB2 at 2.5 µg/ml can bind Herceptin with a linear range of 3.2-80 ng/ml.
ERBB2 / HER2 Protein - Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.5-3 µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
ERBB2 / HER2 Protein
ERBB2 / HER2 Protein - Measured by its binding ability in a functional ELISA. Immobilized human ErbB2 at 2.5 µg/ml can bind Herceptin with a linear range of 3.2-80 ng/ml.
ERBB2 / HER2 Protein - Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.5-3 µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
ERBB2 / HER2 Protein
1 of 3
2 of 3
3 of 3

Human ERBB2 / HER2 Protein (Recombinant Human IgG Fc, C-terminus) (Met1-Thr652) - LS-G47281

Human ERBB2 / HER2 Protein (Recombinant Human IgG Fc, C-terminus) (Met1-Thr652) - LS-G47281

Description:
ERBB2 / HER2 Protein LS-G47281 is a Recombinant Human ERBB2 / HER2 produced in HEK 293 Cells Met1-Thr652 with Human IgG Fc, C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Price
Catalog Number
$441
LS-G47281-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Description:
ERBB2 / HER2 Protein LS-G47281 is a Recombinant Human ERBB2 / HER2 produced in HEK 293 Cells Met1-Thr652 with Human IgG Fc, C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only

Specifications

Type
Recombinant Protein
Target
ERBB2 / HER2
Synonyms
ERBB2 | C-erb B2/neu protein | CD340 | HER2 | Herstatin | MLN 19 | HER-2 | Neu | NGL | Proto-oncogene c-ErbB-2 | Proto-oncogene Neu | CD340 antigen | MLN19 | p185erbB2 | TKR1
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Applications
  • Binding
  • ELISA
  • Functional Assay
  • Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Tag
Human IgG Fc, C-terminus
Region
Met1-Thr652
Predicted Molecular Weight
The recombinant human ErbB2/Fc chimera is a disulfide-linked homodimeric protein generated by proteolytic removal of the signal peptide. The monomer comprises 868 amino acids and has a calculated molecular mass of 96.1 kDa. As a result of glycosylation, the monomer migrates as an approximately 130-140 kDa protein in SDS-PAGE under reducing conditions.
Expression System
HEK 293 Cells
Source Species
Human
Purification
> 90 % as determined by SDS-PAGE
Bio-Activity
Measured by its binding ability in a functional ELISA.Immobilized human ErbB2 at 2.5 µg/ml can bind Herceptin with a linear range of 3.2-80 ng/ml. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.5-3 µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80
Reconstitution
Reconstitute in 400ul of sterile water to a concentration of 0.25mg/ml
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About ERBB2 / HER2
P04626 NM_004448 NP_004439.2

Publications (0)

Customer Reviews (0)

Images

Binding

ERBB2 / HER2 Protein - Measured by its binding ability in a functional ELISA. Immobilized human ErbB2 at 2.5 µg/ml can bind Herceptin with a linear range of 3.2-80 ng/ml.
Measured by its binding ability in a functional ELISA. Immobilized human ErbB2 at 2.5 µg/ml can bind Herceptin with a linear range of 3.2-80 ng/ml.

Proliferation

ERBB2 / HER2 Protein - Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.5-3 µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.5-3 µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.

Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis

ERBB2 / HER2 Protein

Binding

ERBB2 / HER2 Protein - Measured by its binding ability in a functional ELISA. Immobilized human ErbB2 at 2.5 µg/ml can bind Herceptin with a linear range of 3.2-80 ng/ml.
Measured by its binding ability in a functional ELISA. Immobilized human ErbB2 at 2.5 µg/ml can bind Herceptin with a linear range of 3.2-80 ng/ml.

Proliferation

ERBB2 / HER2 Protein - Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.5-3 µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.5-3 µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.

Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis

ERBB2 / HER2 Protein

Binding

ERBB2 / HER2 Protein - Measured by its binding ability in a functional ELISA. Immobilized human ErbB2 at 2.5 µg/ml can bind Herceptin with a linear range of 3.2-80 ng/ml.
Measured by its binding ability in a functional ELISA. Immobilized human ErbB2 at 2.5 µg/ml can bind Herceptin with a linear range of 3.2-80 ng/ml.

Proliferation

ERBB2 / HER2 Protein - Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.5-3 µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.5-3 µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.

Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis

ERBB2 / HER2 Protein

Binding

ERBB2 / HER2 Protein - Measured by its binding ability in a functional ELISA. Immobilized human ErbB2 at 2.5 µg/ml can bind Herceptin with a linear range of 3.2-80 ng/ml.
Measured by its binding ability in a functional ELISA. Immobilized human ErbB2 at 2.5 µg/ml can bind Herceptin with a linear range of 3.2-80 ng/ml.

Proliferation

ERBB2 / HER2 Protein - Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.5-3 µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.5-3 µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.

Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis

ERBB2 / HER2 Protein

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/31/2024