Binding
Measured by its binding ability in a functional ELISA. Immobilized human ErbB2 at 10 µg/mL (100 µL/well) can bind herceptin, the EC50 of herceptin is 7-90 ng/mL.
Proliferation
Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.4-2.4µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Measured by its binding ability in a functional ELISA. Immobilized human ErbB2 at 10 µg/mL (100 µL/well) can bind herceptin, the EC50 of herceptin is 7-90 ng/mL.
Proliferation
Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.4-2.4µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Measured by its binding ability in a functional ELISA. Immobilized human ErbB2 at 10 µg/mL (100 µL/well) can bind herceptin, the EC50 of herceptin is 7-90 ng/mL.
Proliferation
Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.4-2.4µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Measured by its binding ability in a functional ELISA. Immobilized human ErbB2 at 10 µg/mL (100 µL/well) can bind herceptin, the EC50 of herceptin is 7-90 ng/mL.
Proliferation
Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.4-2.4µg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis