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Proteins > DLL4 > LS-G3783
 
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Catalog Number Size Price
LS-G3783-10 10 µg $379 
LS-G3783-50 50 µg $519 
DLL4 Protein - Adipogenesis inhibition of 3T3L-1 cells. 3T3L-1 Cells(mouse pre-adipocyte cells) were maintained in DMEM, supplemented with 10% fetal bovine serum and penicillin-streptomycin. For differentiation of 3T3L-1 Cells, 3T3L-1 Cells were cultured in adipogenic medium which was growth medium supplemented with 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/ml lnsulin (day 0). Medium was changed every 2 days. Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin, and stained with 0.5% filtered Oil Red O in propylene glycol. For negative controls, mouse TNF-alpha (20ng/ml) was added. Recombinant human DLL4-Fc (5 ug/ml) dissolved in DPBS was added to the differentiation medium. These plates were then used to differentiate 3T3L-1 Cells.
DLL4 Protein - Adipogenesis inhibition of MSCs. MSCs (Mesenchymal stem cells) were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin and glutamine. For differentiation of MSCs, MSCs were cultured in adipogenic medium which was growth medium supplemented with 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin, 100 uM Indomethacin (day 1). Medium was changed every 3 days. Staining with Oil Red O was typically performed on day 30. For negative controls, TNF-alpha (20ng/ml) was added. To immobilize Notch ligands on the plastic surface of the culture plates, plates were incubated with a solution of hDLL4-Fc (5 ug/ml) or mCD137-Fc (5 ug/ml) in PBS for 2 hours at 37 degrees C. Plates were then used to differentiate MSCs.
DLL4 Protein - Interaction of human Notch1 with human DLL4. HEK293 cells transfected with a human Notch1 or a human GITR ligand expressing vector were incubated with 25 ug/ml of human GITR-Fc or human DLL4-Fc FITC conjugate for DLL4-Fc binding.
DLL4 Protein - Adipogenesis inhibition of 3T3L-1 cells.
DLL4 Protein - Induction of Hes-1 with the treatment of recombinant human DLL4-Fc (Prod. No. AG-40A-0077). A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 ug/ml of human DLL4-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.
DLL4 Protein - 50 ug of cell lysates derived from hDLL4-Fc or non-treated 3T3L1 cells, which had been either differentiated or undifferentiated, were subjected to western blot by using a mouse adiponectin antibody.
DLL4 Protein - Adipogenesis inhibition of 3T3L-1 cells. 3T3L-1 Cells(mouse pre-adipocyte cells) were maintained in DMEM, supplemented with 10% fetal bovine serum and penicillin-streptomycin. For differentiation of 3T3L-1 Cells, 3T3L-1 Cells were cultured in adipogenic medium which was growth medium supplemented with 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/ml lnsulin (day 0). Medium was changed every 2 days. Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin, and stained with 0.5% filtered Oil Red O in propylene glycol. For negative controls, mouse TNF-alpha (20ng/ml) was added. Recombinant human DLL4-Fc (5 ug/ml) dissolved in DPBS was added to the differentiation medium. These plates were then used to differentiate 3T3L-1 Cells.
DLL4 Protein - Adipogenesis inhibition of MSCs. MSCs (Mesenchymal stem cells) were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin and glutamine. For differentiation of MSCs, MSCs were cultured in adipogenic medium which was growth medium supplemented with 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin, 100 uM Indomethacin (day 1). Medium was changed every 3 days. Staining with Oil Red O was typically performed on day 30. For negative controls, TNF-alpha (20ng/ml) was added. To immobilize Notch ligands on the plastic surface of the culture plates, plates were incubated with a solution of hDLL4-Fc (5 ug/ml) or mCD137-Fc (5 ug/ml) in PBS for 2 hours at 37 degrees C. Plates were then used to differentiate MSCs.
DLL4 Protein - Interaction of human Notch1 with human DLL4. HEK293 cells transfected with a human Notch1 or a human GITR ligand expressing vector were incubated with 25 ug/ml of human GITR-Fc or human DLL4-Fc FITC conjugate for DLL4-Fc binding.
DLL4 Protein - Adipogenesis inhibition of 3T3L-1 cells.
DLL4 Protein - Induction of Hes-1 with the treatment of recombinant human DLL4-Fc (Prod. No. AG-40A-0077). A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 ug/ml of human DLL4-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.
DLL4 Protein - 50 ug of cell lysates derived from hDLL4-Fc or non-treated 3T3L1 cells, which had been either differentiated or undifferentiated, were subjected to western blot by using a mouse adiponectin antibody.
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Human DLL4 Protein (Recombinant Human IgG1 Fc) (aa1-529) - LS-G3783

Human DLL4 Protein (Recombinant Human IgG1 Fc) (aa1-529) - LS-G3783

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Description:
DLL4 Protein LS-G3783 is a Recombinant Human DLL4 produced in HEK 293 Cells aa 1-529 with Human IgG1 Fc tag(s). It is low in endotoxin; Less than 0.01 EU/µg protein (determined by LAL method). For Research Use Only
Price
Catalog Number
$379
LS-G3783-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Publications Reviews Images Popular DLL4 Proteins Request SDS/MSDS

Overview

Description:
DLL4 Protein LS-G3783 is a Recombinant Human DLL4 produced in HEK 293 Cells aa 1-529 with Human IgG1 Fc tag(s). It is low in endotoxin; Less than 0.01 EU/µg protein (determined by LAL method). For Research Use Only

Specifications

Type
Recombinant Protein
Target
DLL4
Synonyms
DLL4 | Delta-like 4 protein | Delta like 4 | Delta-like 4 (Drosophila) | Delta-like 4 homolog | Drosophila Delta homolog 4 | Notch ligand DLL4 | Delta 4 | Delta ligand 4 | Delta-like protein 4 | Delta4 | Hdelta2 | Notch ligand delta-2
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Tag
Human IgG1 Fc
Region
aa 1-529
Predicted Molecular Weight
~80kDa (SDS-PAGE)
Expression System
HEK 293 Cells
Source Species
Human
Purification
Greater than 95% by SDS-PAGE
Bio-Activity
Inhibits adipogenesis of 3T3L-1 cells and mesenchymal stem cells (MSCs). Induces Hes-1 in 3T3L-1 cells.
Endotoxin
Less than 0.01 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from PBS, 0.5% Trehalose
Reconstitution
Reconstitute with 50 µl sterile endotoxin-free water.
Storage
Reconstitute, aliquot and store at -20°C (stable for 6 months at this temperature). Avoid freeze-thaw cycles. PBS containing up to 0.1% BSA should be used for further dilutions.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About DLL4
Q9NR61 NM_019074 NP_061947.1

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Images

Functional Assay

DLL4 Protein - Adipogenesis inhibition of 3T3L-1 cells. 3T3L-1 Cells(mouse pre-adipocyte cells) were maintained in DMEM, supplemented with 10% fetal bovine serum and penicillin-streptomycin. For differentiation of 3T3L-1 Cells, 3T3L-1 Cells were cultured in adipogenic medium which was growth medium supplemented with 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/ml lnsulin (day 0). Medium was changed every 2 days. Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin, and stained with 0.5% filtered Oil Red O in propylene glycol. For negative controls, mouse TNF-alpha (20ng/ml) was added. Recombinant human DLL4-Fc (5 ug/ml) dissolved in DPBS was added to the differentiation medium. These plates were then used to differentiate 3T3L-1 Cells.
Adipogenesis inhibition of 3T3L-1 cells. 3T3L-1 Cells(mouse pre-adipocyte cells) were maintained in DMEM, supplemented with 10% fetal bovine serum and penicillin-streptomycin. For differentiation of 3T3L-1 Cells, 3T3L-1 Cells were cultured in adipogenic medium which was growth medium supplemented with 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/ml lnsulin (day 0). Medium was changed every 2 days. Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin, and stained with 0.5% filtered Oil Red O in propylene glycol. For negative controls, mouse TNF-alpha (20ng/ml) was added. Recombinant human DLL4-Fc (5 ug/ml) dissolved in DPBS was added to the differentiation medium. These plates were then used to differentiate 3T3L-1 Cells.

Functional Assay

DLL4 Protein - Adipogenesis inhibition of MSCs. MSCs (Mesenchymal stem cells) were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin and glutamine. For differentiation of MSCs, MSCs were cultured in adipogenic medium which was growth medium supplemented with 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin, 100 uM Indomethacin (day 1). Medium was changed every 3 days. Staining with Oil Red O was typically performed on day 30. For negative controls, TNF-alpha (20ng/ml) was added. To immobilize Notch ligands on the plastic surface of the culture plates, plates were incubated with a solution of hDLL4-Fc (5 ug/ml) or mCD137-Fc (5 ug/ml) in PBS for 2 hours at 37 degrees C. Plates were then used to differentiate MSCs.
Adipogenesis inhibition of MSCs. MSCs (Mesenchymal stem cells) were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin and glutamine. For differentiation of MSCs, MSCs were cultured in adipogenic medium which was growth medium supplemented with 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin, 100 uM Indomethacin (day 1). Medium was changed every 3 days. Staining with Oil Red O was typically performed on day 30. For negative controls, TNF-alpha (20ng/ml) was added. To immobilize Notch ligands on the plastic surface of the culture plates, plates were incubated with a solution of hDLL4-Fc (5 ug/ml) or mCD137-Fc (5 ug/ml) in PBS for 2 hours at 37 degrees C. Plates were then used to differentiate MSCs.

Functional Assay

DLL4 Protein - Interaction of human Notch1 with human DLL4. HEK293 cells transfected with a human Notch1 or a human GITR ligand expressing vector were incubated with 25 ug/ml of human GITR-Fc or human DLL4-Fc FITC conjugate for DLL4-Fc binding.
Interaction of human Notch1 with human DLL4. HEK293 cells transfected with a human Notch1 or a human GITR ligand expressing vector were incubated with 25 ug/ml of human GITR-Fc or human DLL4-Fc FITC conjugate for DLL4-Fc binding.

Functional Assay

DLL4 Protein - Adipogenesis inhibition of 3T3L-1 cells.
Adipogenesis inhibition of 3T3L-1 cells.

Functional Assay

DLL4 Protein - Induction of Hes-1 with the treatment of recombinant human DLL4-Fc (Prod. No. AG-40A-0077). A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 ug/ml of human DLL4-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.
Induction of Hes-1 with the treatment of recombinant human DLL4-Fc (Prod. No. AG-40A-0077). A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 ug/ml of human DLL4-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.

Functional Assay

DLL4 Protein - Adipogenesis inhibition of 3T3L-1 cells. 3T3L-1 Cells(mouse pre-adipocyte cells) were maintained in DMEM, supplemented with 10% fetal bovine serum and penicillin-streptomycin. For differentiation of 3T3L-1 Cells, 3T3L-1 Cells were cultured in adipogenic medium which was growth medium supplemented with 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/ml lnsulin (day 0). Medium was changed every 2 days. Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin, and stained with 0.5% filtered Oil Red O in propylene glycol. For negative controls, mouse TNF-alpha (20ng/ml) was added. Recombinant human DLL4-Fc (5 ug/ml) dissolved in DPBS was added to the differentiation medium. These plates were then used to differentiate 3T3L-1 Cells.
Adipogenesis inhibition of 3T3L-1 cells. 3T3L-1 Cells(mouse pre-adipocyte cells) were maintained in DMEM, supplemented with 10% fetal bovine serum and penicillin-streptomycin. For differentiation of 3T3L-1 Cells, 3T3L-1 Cells were cultured in adipogenic medium which was growth medium supplemented with 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/ml lnsulin (day 0). Medium was changed every 2 days. Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin, and stained with 0.5% filtered Oil Red O in propylene glycol. For negative controls, mouse TNF-alpha (20ng/ml) was added. Recombinant human DLL4-Fc (5 ug/ml) dissolved in DPBS was added to the differentiation medium. These plates were then used to differentiate 3T3L-1 Cells.

Functional Assay

DLL4 Protein - Adipogenesis inhibition of MSCs. MSCs (Mesenchymal stem cells) were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin and glutamine. For differentiation of MSCs, MSCs were cultured in adipogenic medium which was growth medium supplemented with 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin, 100 uM Indomethacin (day 1). Medium was changed every 3 days. Staining with Oil Red O was typically performed on day 30. For negative controls, TNF-alpha (20ng/ml) was added. To immobilize Notch ligands on the plastic surface of the culture plates, plates were incubated with a solution of hDLL4-Fc (5 ug/ml) or mCD137-Fc (5 ug/ml) in PBS for 2 hours at 37 degrees C. Plates were then used to differentiate MSCs.
Adipogenesis inhibition of MSCs. MSCs (Mesenchymal stem cells) were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin and glutamine. For differentiation of MSCs, MSCs were cultured in adipogenic medium which was growth medium supplemented with 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin, 100 uM Indomethacin (day 1). Medium was changed every 3 days. Staining with Oil Red O was typically performed on day 30. For negative controls, TNF-alpha (20ng/ml) was added. To immobilize Notch ligands on the plastic surface of the culture plates, plates were incubated with a solution of hDLL4-Fc (5 ug/ml) or mCD137-Fc (5 ug/ml) in PBS for 2 hours at 37 degrees C. Plates were then used to differentiate MSCs.

Functional Assay

DLL4 Protein - Interaction of human Notch1 with human DLL4. HEK293 cells transfected with a human Notch1 or a human GITR ligand expressing vector were incubated with 25 ug/ml of human GITR-Fc or human DLL4-Fc FITC conjugate for DLL4-Fc binding.
Interaction of human Notch1 with human DLL4. HEK293 cells transfected with a human Notch1 or a human GITR ligand expressing vector were incubated with 25 ug/ml of human GITR-Fc or human DLL4-Fc FITC conjugate for DLL4-Fc binding.

Functional Assay

DLL4 Protein - Adipogenesis inhibition of 3T3L-1 cells.
Adipogenesis inhibition of 3T3L-1 cells.

Functional Assay

DLL4 Protein - Induction of Hes-1 with the treatment of recombinant human DLL4-Fc (Prod. No. AG-40A-0077). A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 ug/ml of human DLL4-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.
Induction of Hes-1 with the treatment of recombinant human DLL4-Fc (Prod. No. AG-40A-0077). A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 ug/ml of human DLL4-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.

Functional Assay

DLL4 Protein - Adipogenesis inhibition of 3T3L-1 cells. 3T3L-1 Cells(mouse pre-adipocyte cells) were maintained in DMEM, supplemented with 10% fetal bovine serum and penicillin-streptomycin. For differentiation of 3T3L-1 Cells, 3T3L-1 Cells were cultured in adipogenic medium which was growth medium supplemented with 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/ml lnsulin (day 0). Medium was changed every 2 days. Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin, and stained with 0.5% filtered Oil Red O in propylene glycol. For negative controls, mouse TNF-alpha (20ng/ml) was added. Recombinant human DLL4-Fc (5 ug/ml) dissolved in DPBS was added to the differentiation medium. These plates were then used to differentiate 3T3L-1 Cells.
Adipogenesis inhibition of 3T3L-1 cells. 3T3L-1 Cells(mouse pre-adipocyte cells) were maintained in DMEM, supplemented with 10% fetal bovine serum and penicillin-streptomycin. For differentiation of 3T3L-1 Cells, 3T3L-1 Cells were cultured in adipogenic medium which was growth medium supplemented with 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/ml lnsulin (day 0). Medium was changed every 2 days. Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin, and stained with 0.5% filtered Oil Red O in propylene glycol. For negative controls, mouse TNF-alpha (20ng/ml) was added. Recombinant human DLL4-Fc (5 ug/ml) dissolved in DPBS was added to the differentiation medium. These plates were then used to differentiate 3T3L-1 Cells.

Functional Assay

DLL4 Protein - Adipogenesis inhibition of MSCs. MSCs (Mesenchymal stem cells) were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin and glutamine. For differentiation of MSCs, MSCs were cultured in adipogenic medium which was growth medium supplemented with 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin, 100 uM Indomethacin (day 1). Medium was changed every 3 days. Staining with Oil Red O was typically performed on day 30. For negative controls, TNF-alpha (20ng/ml) was added. To immobilize Notch ligands on the plastic surface of the culture plates, plates were incubated with a solution of hDLL4-Fc (5 ug/ml) or mCD137-Fc (5 ug/ml) in PBS for 2 hours at 37 degrees C. Plates were then used to differentiate MSCs.
Adipogenesis inhibition of MSCs. MSCs (Mesenchymal stem cells) were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin and glutamine. For differentiation of MSCs, MSCs were cultured in adipogenic medium which was growth medium supplemented with 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin, 100 uM Indomethacin (day 1). Medium was changed every 3 days. Staining with Oil Red O was typically performed on day 30. For negative controls, TNF-alpha (20ng/ml) was added. To immobilize Notch ligands on the plastic surface of the culture plates, plates were incubated with a solution of hDLL4-Fc (5 ug/ml) or mCD137-Fc (5 ug/ml) in PBS for 2 hours at 37 degrees C. Plates were then used to differentiate MSCs.

Functional Assay

DLL4 Protein - Interaction of human Notch1 with human DLL4. HEK293 cells transfected with a human Notch1 or a human GITR ligand expressing vector were incubated with 25 ug/ml of human GITR-Fc or human DLL4-Fc FITC conjugate for DLL4-Fc binding.
Interaction of human Notch1 with human DLL4. HEK293 cells transfected with a human Notch1 or a human GITR ligand expressing vector were incubated with 25 ug/ml of human GITR-Fc or human DLL4-Fc FITC conjugate for DLL4-Fc binding.

Functional Assay

DLL4 Protein - Adipogenesis inhibition of 3T3L-1 cells.
Adipogenesis inhibition of 3T3L-1 cells.

Functional Assay

DLL4 Protein - Induction of Hes-1 with the treatment of recombinant human DLL4-Fc (Prod. No. AG-40A-0077). A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 ug/ml of human DLL4-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.
Induction of Hes-1 with the treatment of recombinant human DLL4-Fc (Prod. No. AG-40A-0077). A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 ug/ml of human DLL4-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.

Functional Assay

DLL4 Protein - Adipogenesis inhibition of 3T3L-1 cells. 3T3L-1 Cells(mouse pre-adipocyte cells) were maintained in DMEM, supplemented with 10% fetal bovine serum and penicillin-streptomycin. For differentiation of 3T3L-1 Cells, 3T3L-1 Cells were cultured in adipogenic medium which was growth medium supplemented with 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/ml lnsulin (day 0). Medium was changed every 2 days. Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin, and stained with 0.5% filtered Oil Red O in propylene glycol. For negative controls, mouse TNF-alpha (20ng/ml) was added. Recombinant human DLL4-Fc (5 ug/ml) dissolved in DPBS was added to the differentiation medium. These plates were then used to differentiate 3T3L-1 Cells.
Adipogenesis inhibition of 3T3L-1 cells. 3T3L-1 Cells(mouse pre-adipocyte cells) were maintained in DMEM, supplemented with 10% fetal bovine serum and penicillin-streptomycin. For differentiation of 3T3L-1 Cells, 3T3L-1 Cells were cultured in adipogenic medium which was growth medium supplemented with 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/ml lnsulin (day 0). Medium was changed every 2 days. Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin, and stained with 0.5% filtered Oil Red O in propylene glycol. For negative controls, mouse TNF-alpha (20ng/ml) was added. Recombinant human DLL4-Fc (5 ug/ml) dissolved in DPBS was added to the differentiation medium. These plates were then used to differentiate 3T3L-1 Cells.

Functional Assay

DLL4 Protein - Adipogenesis inhibition of MSCs. MSCs (Mesenchymal stem cells) were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin and glutamine. For differentiation of MSCs, MSCs were cultured in adipogenic medium which was growth medium supplemented with 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin, 100 uM Indomethacin (day 1). Medium was changed every 3 days. Staining with Oil Red O was typically performed on day 30. For negative controls, TNF-alpha (20ng/ml) was added. To immobilize Notch ligands on the plastic surface of the culture plates, plates were incubated with a solution of hDLL4-Fc (5 ug/ml) or mCD137-Fc (5 ug/ml) in PBS for 2 hours at 37 degrees C. Plates were then used to differentiate MSCs.
Adipogenesis inhibition of MSCs. MSCs (Mesenchymal stem cells) were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin and glutamine. For differentiation of MSCs, MSCs were cultured in adipogenic medium which was growth medium supplemented with 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin, 100 uM Indomethacin (day 1). Medium was changed every 3 days. Staining with Oil Red O was typically performed on day 30. For negative controls, TNF-alpha (20ng/ml) was added. To immobilize Notch ligands on the plastic surface of the culture plates, plates were incubated with a solution of hDLL4-Fc (5 ug/ml) or mCD137-Fc (5 ug/ml) in PBS for 2 hours at 37 degrees C. Plates were then used to differentiate MSCs.

Functional Assay

DLL4 Protein - Interaction of human Notch1 with human DLL4. HEK293 cells transfected with a human Notch1 or a human GITR ligand expressing vector were incubated with 25 ug/ml of human GITR-Fc or human DLL4-Fc FITC conjugate for DLL4-Fc binding.
Interaction of human Notch1 with human DLL4. HEK293 cells transfected with a human Notch1 or a human GITR ligand expressing vector were incubated with 25 ug/ml of human GITR-Fc or human DLL4-Fc FITC conjugate for DLL4-Fc binding.

Functional Assay

DLL4 Protein - Adipogenesis inhibition of 3T3L-1 cells.
Adipogenesis inhibition of 3T3L-1 cells.

Functional Assay

DLL4 Protein - Induction of Hes-1 with the treatment of recombinant human DLL4-Fc (Prod. No. AG-40A-0077). A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 ug/ml of human DLL4-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.
Induction of Hes-1 with the treatment of recombinant human DLL4-Fc (Prod. No. AG-40A-0077). A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5 ug/ml of human DLL4-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.

Popular DLL4 Proteins

DLL4 Protein - Adipogenesis inhibition of 3T3L1 cells. 3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml mDLL4-Fc (Prod. No. AG-40A-0145), hTNFalpha and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.
Mouse DLL4 Protein (Recombinant Human IgG1 Fc) (aa1-532) - LS-G3829
Source: Human
Tag: Human IgG1 Fc
DLL4 Protein - Recombinant Delta Like Protein 4 (dLL4) by SDS-PAGE
Human DLL4 Protein (Recombinant His, N-Terminal) (Gly453-Arg654) - LS-G132976
Source: E. coli
Tag: His, N-Terminal
Product
Human DLL4 Protein (Recombinant) - LS-G127
Source: Human
Tag: None

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Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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