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Catalog Number Size Price
LS-B16077-50 50 µl (1 mg/ml) $460 
KRT8 / CK8 / Cytokeratin 8 Antibody - Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
KRT8 / CK8 / Cytokeratin 8 Antibody - Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
KRT8 / CK8 / Cytokeratin 8 Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
KRT8 / CK8 / Cytokeratin 8 Antibody - Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
KRT8 / CK8 / Cytokeratin 8 Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
KRT8 / CK8 / Cytokeratin 8 Antibody - Immunofluorescent analysis of Cytokeratin 8 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
KRT8 / CK8 / Cytokeratin 8 Antibody - Western blot analysis of Cytokeratin 8 expression in HeLa (A); NIH3T3 (B); H9C2 (C); MCF7 (D) whole cell lysates.
KRT8 / CK8 / Cytokeratin 8 Antibody - Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
KRT8 / CK8 / Cytokeratin 8 Antibody - Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
KRT8 / CK8 / Cytokeratin 8 Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
KRT8 / CK8 / Cytokeratin 8 Antibody - Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
KRT8 / CK8 / Cytokeratin 8 Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
KRT8 / CK8 / Cytokeratin 8 Antibody - Immunofluorescent analysis of Cytokeratin 8 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
KRT8 / CK8 / Cytokeratin 8 Antibody - Western blot analysis of Cytokeratin 8 expression in HeLa (A); NIH3T3 (B); H9C2 (C); MCF7 (D) whole cell lysates.
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PathPlus™ Polyclonal Rabbit anti‑Human KRT8 / CK8 / Cytokeratin 8 Antibody (C‑Terminus, IHC, IF, WB) LS‑B16077

PathPlus™ Polyclonal Rabbit anti‑Human KRT8 / CK8 / Cytokeratin 8 Antibody (C‑Terminus, IHC, IF, WB) LS‑B16077

Note: This antibody replaces LS-C352438
Antibody:
KRT8 / CK8 / Cytokeratin 8 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B16077-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
KRT8 / CK8 / Cytokeratin 8 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
Cytokeratin 8 (KRT8, CK8) is a Type II intermediate filament chain keratin that is expressed in simple glandular epithelia and the liver. Along with KRT18, KRT8 protects the liver from stress-induced injury and apoptosis. Mutations in this gene have been linked to cryptogenic cirrhosis, rhabdoid tumors, chronic pancreatitis and liver disease. It is a component of nuclear inclusions in rhaboid tumors and Mallory bodies in liver diseases. KRT8 is positive in a variety of cancers. References: Hepatology 2007 46(5):1639; Mod Pathol 2002 15:146; Dig Liver dis 2003 35:416; J Cell Biol 2005 171(6):931
Target
Human KRT8 / CK8 / Cytokeratin 8
Synonyms
KRT8 | CARD2 | CK-8 | CYK8 | Cytokeratin-8 | CK8 | K2C8 | K8 | KO | Keratin-8 | Type-II keratin Kb8 | Cytokeratin 8 | Keratin 8
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human Cytokeratin 8.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of Cytokeratin 8 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About KRT8 / CK8 / Cytokeratin 8
P05787 NM_002273 NP_002264.1

Validation

KRT8 / CK8 / Cytokeratin 8 Antibody - Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)

KRT8 / CK8 / Cytokeratin 8 Antibody - Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE)

KRT8 / CK8 / Cytokeratin 8 Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

KRT8 / CK8 / Cytokeratin 8 Antibody - Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)

KRT8 / CK8 / Cytokeratin 8 Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

PathPlus™ KRT8 / CK8 / Cytokeratin 8 Antibody (C-Terminus) for IHC, ICC, IF/Immunofluorescence, WB/Western LS-B16077 has an LSBio Rating of
Laboratory Validation Score (5)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/31/2024