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Catalog Number Size Price
LS-C681098-50 50 µg $294 
LS-C681098-100 100 µg $360 
POU5F1B Antibody - Immunohistochemistry Dilution at 1:300 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
POU5F1B Antibody - Immunohistochemistry Dilution at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
POU5F1B Antibody - Western Blot Positive WB detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate All Lanes: POU5F1B antibody at 6.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39 KDa Observed band size: 39 KDa
POU5F1B Antibody - Immunohistochemistry Dilution at 1:300 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
POU5F1B Antibody - Immunohistochemistry Dilution at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
POU5F1B Antibody - Western Blot Positive WB detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate All Lanes: POU5F1B antibody at 6.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39 KDa Observed band size: 39 KDa
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Polyclonal Rabbit anti‑Human POU5F1B Antibody (IHC, WB) LS‑C681098

Polyclonal Rabbit anti‑Human POU5F1B Antibody (IHC, WB) LS‑C681098

Antibody:
POU5F1B Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$294
LS-C681098-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
POU5F1B Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
POU5F1B antibody LS-C681098 is an unconjugated rabbit polyclonal antibody to human POU5F1B. Validated for ELISA, IHC and WB.
Target
Human POU5F1B
Synonyms
POU5F1B | OCT4-PG1 | POU 5 domain protein | POU5F1P1 | POU5FLC20 | POU class 5 homeobox 1B | POU5F1P4 | POU5FLC8 | Octamer-binding protein 3-like | OTF3C | OTF3P1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, Biotin, HRP.
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant Human Putative POU domain, class 5, transcription factor 1B protein (10-138AA)
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
0.01 M PBS, pH 7.4, 0.03% ProClin™ 300, 50% Glycerol
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About POU5F1B
Q06416 NP_001153014.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/27/2024