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Antibodies > IKBKG / NEMO / IKK Gamma > LS-C801242
 
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Catalog Number Size Price
LS-C801242-100 100 µl (1 mg/ml) $379 
LS-C801242-200 200 µl (1 mg/ml) $421 
IKBKG / NEMO / IKK Gamma Antibody - 1:200 staining human Testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
IKBKG / NEMO / IKK Gamma Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
IKBKG / NEMO / IKK Gamma Antibody - Western blot analysis of IKK-gamma phosphorylation expression in TNF-a treated 293 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
IKBKG / NEMO / IKK Gamma Antibody - Western blot analysis of Phospho-IKK gamma (Ser31) antibody expression in TNF-a treated 293 cells lysates. The lane on the right is treated with the antigen-specific peptide.
IKBKG / NEMO / IKK Gamma Antibody - 1:200 staining human Testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
IKBKG / NEMO / IKK Gamma Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
IKBKG / NEMO / IKK Gamma Antibody - Western blot analysis of IKK-gamma phosphorylation expression in TNF-a treated 293 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
IKBKG / NEMO / IKK Gamma Antibody - Western blot analysis of Phospho-IKK gamma (Ser31) antibody expression in TNF-a treated 293 cells lysates. The lane on the right is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human IKBKG / NEMO / IKK Gamma Antibody (phospho‑Ser31, IHC, IF, WB) LS‑C801242

Polyclonal Rabbit anti‑Human IKBKG / NEMO / IKK Gamma Antibody (phospho‑Ser31, IHC, IF, WB) LS‑C801242

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Antibody:
IKBKG / NEMO / IKK Gamma Rabbit anti-Human Polyclonal (pSer31) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801242-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
IKBKG / NEMO / IKK Gamma Rabbit anti-Human Polyclonal (pSer31) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
IKK Gamma antibody LS-C801242 is an unconjugated rabbit polyclonal antibody to human IKK Gamma (IKBKG / NEMO) (pSer31). Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human IKBKG / NEMO / IKK Gamma
Synonyms
IKBKG | AMCBX1 | FIP3 | Fip3p | IkappaB kinase gamma | IKKgamma | IKK-gamma | NEMO | NF-kappa-B essential modifier | I-kappa-B kinase gamma-subunit | I-kappa-B kinase subunit gamma | IkB kinase gamma subunit | IKKAP1 | Incontinentia pigmenti | IP2 | IPD2 | NF-kappa-B essential modulator | ZC2HC9 | FIP-3 | IkB kinase subunit gamma | IKKG | IP1 | NFkappaB essential modulator
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human IKK- gamma around the phosphorylation site of Serine 31.
Epitope
pSer31
Specificity
Phospho-IKK- gamma (Ser31) Antibody detects endogenous levels of IKK- gamma only when phosphorylated at Serine 31.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IKBKG / NEMO / IKK Gamma
Q9Y6K9 NM_003639 NP_003630.1

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IKBKG / NEMO / IKK Gamma Antibody - Immunohistochemical analysis of IKK gamma (pS85) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
IKBKG / NEMO / IKK Gamma Antibody (phospho‑Ser85) LS‑C353075
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
IKBKG / NEMO / IKK Gamma Antibody - Immunohistochemical analysis of IKK gamma staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
IKBKG / NEMO / IKK Gamma Antibody (Internal) LS‑C359024
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, Immunoprecipitation
IKBKG / NEMO / IKK Gamma Antibody - Immunohistochemical analysis of IKK gamma (pS31) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
IKBKG / NEMO / IKK Gamma Antibody (phospho‑Ser31) LS‑C353080
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
See all IKBKG / NEMO / IKK Gamma Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/27/2024
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