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Catalog Number	Size	Price
LS-C662074-10	10 µg	$318
LS-C662074-100	100 µg	$470

CHAT Antibody - IHC analysis of CHAT using anti-CHAT antibody. CHAT was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CHAT Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

CHAT Antibody - IHC analysis of CHAT using anti-CHAT antibody. CHAT was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-CHAT Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

CHAT Antibody - Western blot analysis of CHAT using anti-CHAT antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHAT antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CHAT at approximately 83KD. The expected band size for CHAT is at 83KD.

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Polyclonal Rabbit anti‑Human CHAT Antibody (IHC, WB) LS‑C662074

Name: Anti-CHAT Antibody | Rabbit anti-Human Polyclonal IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C662074-10
Price: 318.0000 USD
Availability: InStock

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Antibody:

CHAT Rabbit anti-Human Polyclonal Antibody

Application:

IHC, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

$318

LS-C662074-10

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

CHAT Rabbit anti-Human Polyclonal Antibody

Application:

IHC, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

CHAT antibody LS-C662074 is an unconjugated rabbit polyclonal antibody to human CHAT. Validated for IHC and WB.

Target

Human CHAT

Synonyms

See All CHAT Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E.coli-derived human CHAT recombinant protein (Position: D446-R652). Human CHAT shares 88.4% and 87% amino acid (aa) sequence identity with mouse and rat CHAT, respectively.

Applications

IHC
Western blot

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About CHAT

Uniprot: P28329 NCBI: NM_020549 NP_065574.3