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Antibodies > ATP1A1 > LS-C800943
 
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Catalog Number Size Price
LS-C800943-100 100 µl (1 mg/ml) $379 
LS-C800943-200 200 µl (1 mg/ml) $421 
ATP1A1 Antibody - 1/100 staining human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
ATP1A1 Antibody - 1:100 staining mouse intestine tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ATP1A1 Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
ATP1A1 Antibody - Western blot analysis of ATPase phosphorylation expression in PMA treated 293 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
ATP1A1 Antibody - Western blot analysis of Phospho-ATPase (Ser16) expression in various lysates
ATP1A1 Antibody - 1/100 staining human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
ATP1A1 Antibody - 1:100 staining mouse intestine tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ATP1A1 Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
ATP1A1 Antibody - Western blot analysis of ATPase phosphorylation expression in PMA treated 293 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
ATP1A1 Antibody - Western blot analysis of Phospho-ATPase (Ser16) expression in various lysates
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Polyclonal Rabbit anti‑Human ATP1A1 Antibody (phospho‑Ser16, IHC, IF, WB) LS‑C800943

Polyclonal Rabbit anti‑Human ATP1A1 Antibody (phospho‑Ser16, IHC, IF, WB) LS‑C800943

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Antibody:
ATP1A1 Rabbit anti-Human Polyclonal (pSer16) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800943-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ATP1A1 Rabbit anti-Human Polyclonal (pSer16) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
ATP1A1 antibody LS-C800943 is an unconjugated rabbit polyclonal antibody to ATP1A1 (pSer16) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human ATP1A1
Synonyms
ATP1A1 | Na+, K+ ATPase alpha subunit | Na,K-ATPase alpha-1 subunit | Na+/K+ ATPase 1 | Sodium pump 1 | Sodium pump subunit alpha-1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human ATPase around the phosphorylation site of Serine 16.
Epitope
pSer16
Specificity
Phospho-ATPase (Ser16) Antibody detects endogenous levels of ATPase only when phosphorylated at Serine 16.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ATP1A1
P05023 NM_000701 NP_000692.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 10/31/2024
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