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Catalog Number Size Price
LS-C287184-10 10 µl $295 
LS-C287184-100 100 µl (1 mg/ml) $514 
TP53 / p53 Antibody - Detection of human p53 by immunohistochemistry. Sample: FFPE section of human lung cancer Antibody: Affinity purified rabbit anti- p53 used at 1:5,000 (0.2µg/ml). Detection: DAB
TP53 / p53 Antibody - Detection of Human p53 by Immunohistochemistry. Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti-p53 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
TP53 / p53 Antibody - Detection of human P53 by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-P53 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
TP53 / p53 Antibody - Detection of Human p53 by Western Blot. Samples: Whole cell lysate (100 ug) from 293T cells that were treated with an siRNA for p53 (+) or mock treated (-). Antibody: Rabbit anti-p53 antibody used at the indicated concentrations. Detection: Chemiluminescence with an exposure time of less than 1 minute.
TP53 / p53 Antibody - Flow Cytometrical Analysis of p53 in Jurkat Cells. Jurkat cells were fixed in 1.5% PFA, and permeabilized in 90% Methanol. One million cells were stained with 1.5 ug anti-KLH IgG control or anti-p53 and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Anti-KLH control IgG (black); anti-p53 (red).
TP53 / p53 Antibody - Detection of human p53 by immunohistochemistry. Sample: FFPE section of human lung cancer Antibody: Affinity purified rabbit anti- p53 used at 1:5,000 (0.2µg/ml). Detection: DAB
TP53 / p53 Antibody - Detection of Human p53 by Immunohistochemistry. Sample: FFPE section of human skin carcinoma. Antibody: Affinity purified rabbit anti-p53 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.
TP53 / p53 Antibody - Detection of human P53 by western blot. Samples: Whole cell lysate (5, 15 and 50 µg) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-P53 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
TP53 / p53 Antibody - Detection of Human p53 by Western Blot. Samples: Whole cell lysate (100 ug) from 293T cells that were treated with an siRNA for p53 (+) or mock treated (-). Antibody: Rabbit anti-p53 antibody used at the indicated concentrations. Detection: Chemiluminescence with an exposure time of less than 1 minute.
TP53 / p53 Antibody - Flow Cytometrical Analysis of p53 in Jurkat Cells. Jurkat cells were fixed in 1.5% PFA, and permeabilized in 90% Methanol. One million cells were stained with 1.5 ug anti-KLH IgG control or anti-p53 and secondary FITC-conjugated goat anti-rabbit (in a 150ul reaction). Anti-KLH control IgG (black); anti-p53 (red).
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Polyclonal Rabbit anti‑Human TP53 / p53 Antibody (aa50‑100, IHC, WB) LS‑C287184

Polyclonal Rabbit anti‑Human TP53 / p53 Antibody (aa50‑100, IHC, WB) LS‑C287184

Antibody:
TP53 / p53 Rabbit anti-Human Polyclonal (aa50-100) Antibody
Application:
IHC, WB, IP, Flo, ChIP-Seq
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$295
LS-C287184-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
TP53 / p53 Rabbit anti-Human Polyclonal (aa50-100) Antibody
Application:
IHC, WB, IP, Flo, ChIP-Seq
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
P53 antibody LS-C287184 is an unconjugated rabbit polyclonal antibody to human p53 (TP53) (aa50-100). Validated for ChIP-Seq, Flow, IHC, IP and WB.
Target
Human TP53 / p53
Synonyms
TP53 | BCC7 | Cellular tumor antigen p53 | p53 | TRP53 | p53 tumor suppressor | Phosphoprotein p53 | Antigen NY-CO-13 | Tumor protein p53 | Tumor suppressor p53 | LFS1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
A portion of GeneID: 7157 corresponding to amino acids between 50 and 100 of UniprotID: P04637
Epitope
aa50-100
Specificity
Region between residues 50 and 100 of human tumor protein p53 using the numbering given in SwissProt entry P04637 (GeneID 7157).
Applications
  • IHC (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:10000)
  • Immunoprecipitation (2 - 5 µg/mg lysate)
  • Flow Cytometry
  • ChIP-sequencing
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. ChIP-Seq: 4 µg/30 µg chromatin Flo: 1.5 µg per 1 X 10^6 cells in a 150 µl volume
Manufacturer
Bethyl Laboratories, Inc.
Presentation
Tris-citrate/phosphate buffer, pH 7-8, 0.09% sodium azide.
Storage
Store at 2-8°C for up to one year.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TP53 / p53
P04637 NM_000546 NP_000537.3

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Requested From: United States
Date Requested: 4/20/2024