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Antibodies > TERT / Telomerase > LS-B11086
 
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Catalog Number Size Price
LS-B11086-200 200 µl (0.5 mg/ml) $460 
TERT / Telomerase Antibody - Anti-TERT / Telomerase antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
TERT / Telomerase Antibody - Fluorescent confocal image of HeLa cell stained with TERT Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TERT primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TERT immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.
TERT / Telomerase Antibody - Fluorescent confocal image of SY5Y cells stained with TERT antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated TERT primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). TERT immunoreactivity is localized to the nucleus of SY5Y cells.
TERT / Telomerase Antibody - Western blot analysis of Anti-TERT antibody (LS-B11086, 1:2000 dilution; 20 µg lysates/proteins per lane). Lane 1: HeLa whole cell lysate. Lane 2: HT-29 whole cell lysate. Lane 3: Jurkat whole cell lysate. Lane 4: MOLT-4 whole cell lysate. Lane 5: PC-3 whole cell lysate. Lane 6: 293 whole cell lysate. Lane 7: COLO 205 whole cell lysate. Antibody produced bands at ~127 kDa.
TERT / Telomerase Antibody - TERT Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
TERT / Telomerase Antibody - Anti-TERT / Telomerase antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
TERT / Telomerase Antibody - Fluorescent confocal image of HeLa cell stained with TERT Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TERT primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TERT immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.
TERT / Telomerase Antibody - Fluorescent confocal image of SY5Y cells stained with TERT antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated TERT primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). TERT immunoreactivity is localized to the nucleus of SY5Y cells.
TERT / Telomerase Antibody - Western blot analysis of Anti-TERT antibody (LS-B11086, 1:2000 dilution; 20 µg lysates/proteins per lane). Lane 1: HeLa whole cell lysate. Lane 2: HT-29 whole cell lysate. Lane 3: Jurkat whole cell lysate. Lane 4: MOLT-4 whole cell lysate. Lane 5: PC-3 whole cell lysate. Lane 6: 293 whole cell lysate. Lane 7: COLO 205 whole cell lysate. Antibody produced bands at ~127 kDa.
TERT / Telomerase Antibody - TERT Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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IHC‑plus™ Polyclonal Rabbit anti‑Human TERT / Telomerase Antibody (aa627‑656, IHC, IF, WB) LS‑B11086

IHC‑plus™ Polyclonal Rabbit anti‑Human TERT / Telomerase Antibody (aa627‑656, IHC, IF, WB) LS‑B11086

IHC Validated
(5.0) Rating
Submit Review (1) Publication(s) LSBio Guarantee
Note: This antibody replaces LS-C98109
Antibody:
TERT / Telomerase Rabbit anti-Human Polyclonal (aa627-656) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B11086-200
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
TERT / Telomerase Rabbit anti-Human Polyclonal (aa627-656) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Telomerase antibody LS-B11086 is an unconjugated rabbit polyclonal antibody to human Telomerase (TERT) (aa627-656). Validated for Flow, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
(1) Publication(s)
Target
Human TERT / Telomerase
Synonyms
TERT | DKCB4 | EST2 | HEST2 | PFBMFT1 | Telomerase | TP2 | DKCA2 | HTRT | TCS1 | Telomerase catalytic subunit | TRT
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Epitope
aa627-656
Specificity
This TERT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 627-656 amino acids from the Central region of human TERT.
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:2000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TERT / Telomerase
O14746 AY007685 AAG23289.1

Validation

TERT / Telomerase Antibody - Anti-TERT / Telomerase antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-TERT / Telomerase antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-TERT / Telomerase antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
See More About...
About LSBio Validation process

LSBio Ratings

IHC-plus™ TERT / Telomerase Antibody (aa627-656) for IHC, IF/Immunofluorescence, WB/Western, Flow LS-B11086 has an LSBio Rating of
(5.0) Rating
Publications (4)
Laboratory Validation Score (4)

Publications (1)

MicroRNA-22 enhances radiosensitivity in cervical cancer cell lines via direct inhibition of c-Myc binding protein, and the subsequent reduction in hTERT expression. Mayumi Nakamura, Masami Hayashi, Hiromi Konishi, Misa Nunode, Keisuke Ashihara, Hiroshi Sasaki, Yoshito Terai, Masahide Ohmichi. Oncology letters. 2020 Mar;19:2213-2222.
Full Text Article PubMed: 32194719 PMC: PMC7038919
Submit a Publication for LS-B11086

Customer Reviews (0)

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TERT / Telomerase Antibody - Immunofluorescence analysis of HUVEC cells, using Telomerase Antibody. The picture on the right is blocked with the synthesized peptide.
TERT / Telomerase Antibody (aa196‑245) LS‑C118018
Species: Human
Applications: Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay
TERT / Telomerase ELISA Kit
Human TERT / Telomerase (Sandwich ELISA) ELISA Kit - LS-F7351
Reactivity: Human
Range: 15.63-1000 pg/ml
See all TERT / Telomerase Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/20/2024
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