RAG2 / RAG-2

recombination activating gene 2

Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. DNA cleavage by the RAG complex occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3.

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Antibodies (17)

ELISA Kits (10)

Proteins (5)

Type

Custom (2)

Over-Expression Lysate (2)

Primary (17)

Recombinant (3)

Sandwich (8)

Target

RAG2 / RAG-2 (32)

Reactivity

Human (20)

Mouse (8)

Rat (4)

Bovine (3)

Chicken (2)

Dog (3)

Gibbon (3)

Guinea pig (1)

Monkey (1)

Pig (3)

Porcine (1)

Rabbit (1)

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Application

IHC (4)

IHC-P (3)

WB (16)

Flo (1)

ELISA (4)

ICC (2)

IF (3)

IP (1)

Host

rabbit (13)

mouse (4)

Isotype

IgG (7)

IgG1 (3)

IgG2b,k (1)

Clonality

monoclonal mc (4)

polyclonal pc (13)

Clone

2G8 (1)

4D5 (2)

Format

96-Well Strip Plate (10)

Biotin Conjugated (1)

FITC Conjugated (1)

HRP Conjugated (1)

Unconjugated (14)

Epitope

C-Terminus (4)

Amino Acid: 350-527 (1)

aa271-527 (1)

aa498-527 (1)

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No (32)

Sample Type

Plasma (3)

Serum (3)

Tissue Homogenates (1)

Tag

GST (1)

GST, N-terminus (1)

GST, N-terminus + His, C-terminus (1)

Myc-DDK (Flag) (2)

Species

Human (4)

Source

293T Cells (1)

E. coli (1)

HEK 293 Cells (1)

Wheat Germ Extract (2)

Purification

Purified (1)

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RAG2 / RAG-2 Antibody (aa498-527) LS-C408005

Antibody:

RAG2 / RAG-2 Rabbit anti-Human Polyclonal (aa498-527) Antibody

Reactivity:

Mouse, Rat, Human

Applications:

IHC, IHC-P, WB

Format:

Unconjugated

Price:

10 µg/$318; 100 µg/$470

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RAG2 / RAG-2 Antibody (clone 4D5) LS-C109145

Antibody:

RAG2 / RAG-2 Mouse anti-Human Monoclonal (4D5) Antibody

Reactivity:

Human

Applications:

ELISA, WB

Format:

Unconjugated

Price:

100 µl/$403

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RAG2 / RAG-2 Antibody LS-C752923

Antibody:

RAG2 / RAG-2 Rabbit anti-Human Polyclonal Antibody

Reactivity:

Human

Applications:

ELISA, Flo, IF, IHC, IP, WB

Format:

Unconjugated

Price:

100 µg/$341

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RAG2 / RAG-2 Antibody (C-Terminus) LS-C145992

Antibody:

RAG2 / RAG-2 Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Reactivity:

Human

Applications:

WB

Format:

Unconjugated

Price:

100 µl/$424

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RAG2 / RAG-2 Antibody (C-Terminus, HRP) LS-C468307

Antibody:

RAG2 / RAG-2 Rabbit anti-Human Polyclonal (C-Terminus) (HRP) Antibody

Reactivity:

Dog, Bovine, Pig, Gibbon, Human

Applications:

WB

Format:

HRP Conjugated

Price:

100 µl/$469

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RAG2 / RAG-2 Antibody (C-Terminus, Biotin) LS-C468305

Antibody:

RAG2 / RAG-2 Rabbit anti-Human Polyclonal (C-Terminus) (Biotin) Antibody

Reactivity:

Dog, Bovine, Pig, Gibbon, Human

Applications:

WB

Format:

Biotin Conjugated

Price:

100 µl/$469

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RAG2 / RAG-2 Antibody (C-Terminus, FITC) LS-C468306

Antibody:

RAG2 / RAG-2 Rabbit anti-Human Polyclonal (C-Terminus) (FITC) Antibody

Reactivity:

Dog, Bovine, Pig, Gibbon, Human

Applications:

WB

Format:

FITC Conjugated

Price:

100 µl/$469

• Unconjugated
• HRP
• Biotin
• FITC

• Unconjugated
• HRP
• Biotin
• More...
  • FITC

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RAG2 / RAG-2 Antibody (aa271-527) LS-C186092

Antibody:

RAG2 / RAG-2 Rabbit anti-Human Polyclonal (aa271-527) Antibody

Reactivity:

Human

Applications:

ICC, IHC, IHC-P, WB

Format:

Unconjugated

Price:

100 µl/$450

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RAG2 / RAG-2 Antibody LS-C490468

Antibody:

RAG2 / RAG-2 Rabbit anti-Human Polyclonal Antibody

Reactivity:

Mouse, Rat, Human

Applications:

IHC, IHC-P, WB

Format:

Unconjugated

Price:

100 µg/$575

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RAG2 / RAG-2 Antibody LS-C482475

Antibody:

RAG2 / RAG-2 Rabbit anti-Human Polyclonal Antibody

Reactivity:

Mouse, Rat, Human

Applications:

ICC, IF, WB

Format:

Unconjugated

Price:

50 µl/$286; 100 µl/$321; 200 µl/$386

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RAG2 / RAG-2 Antibody LS-C812757

Antibody:

RAG2 / RAG-2 Mouse anti-Human Monoclonal Antibody

Reactivity:

Human

Applications:

ELISA, WB

Format:

Unconjugated

Price:

50 µg/$332; 100 µg/$404; 200 µg/$505

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RAG2 / RAG-2 Antibody (clone 2G8) LS-C198057

Antibody:

RAG2 / RAG-2 Mouse anti-Human Monoclonal (2G8) Antibody

Reactivity:

Rat, Human

Applications:

ELISA, WB

Format:

Unconjugated

Price:

100 µg/$423

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RAG2 / RAG-2 Antibody LS-C834301

Antibody:

RAG2 / RAG-2 Rabbit anti-Human Polyclonal Antibody

Reactivity:

Mouse, Human

Applications:

IF

Format:

Unconjugated

Price:

60 µl/$331; 120 µl/$403; 200 µl/$529

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RAG2 / RAG-2 Antibody (Amino Acid: 350-527, clone 4D5) LS-C758609

Antibody:

RAG2 / RAG-2 Mouse anti-Human Monoclonal (Amino Acid: 350-527) (4D5) Antibody

Reactivity:

Human

Applications:

WB

Format:

Unconjugated

Price:

100 µl/$518

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RAG2 / RAG-2 Antibody LS-C334173

Antibody:

RAG2 / RAG-2 Rabbit anti-Human Polyclonal Antibody

Reactivity:

Mouse, Human

Applications:

WB

Format:

Unconjugated

Price:

20 µl/$287; 50 µl/$341; 100 µl/$431; 200 µl/$539

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RAG2 / RAG-2 Antibody LS-C747587

Antibody:

RAG2 / RAG-2 Rabbit anti-Human Polyclonal Antibody

Reactivity:

Mouse, Human

Applications:

WB

Format:

Unconjugated

Price:

20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503

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RAG2 / RAG-2 Antibody LS-C832967

Antibody:

RAG2 / RAG-2 Rabbit anti-Human Polyclonal Antibody

Reactivity:

Mouse, Human

Applications:

WB

Format:

Unconjugated

Price:

60 µl/$331; 120 µl/$403; 200 µl/$529

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Rabbit RAG2 / RAG-2 (Sandwich ELISA) ELISA Kit - LS-F47684

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Rabbit

Range:

0.625 - 40 pg/ml

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$728

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Guinea pig RAG2 / RAG-2 (Sandwich ELISA) ELISA Kit - LS-F47677

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Guinea pig

Range:

0.781 - 50 pg/ml

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$728

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Porcine RAG2 / RAG-2 (Sandwich ELISA) ELISA Kit - LS-F47679

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Porcine

Range:

15.625 - 1000 pg/ml

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$728

Select

Chicken RAG2 / RAG-2 (Sandwich ELISA) ELISA Kit - LS-F47681

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Chicken

Range:

0.781 - 50 pg/ml

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$728

Select

Monkey RAG2 / RAG-2 (Sandwich ELISA) ELISA Kit - LS-F47678

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Monkey

Range:

15.625 - 1000 pg/ml

Detection:

Colorimetric - 450nm (TMB)

Price:

1 Plate/$728

Select

Human RAG2 / RAG-2 Protein (Recombinant GST, N-terminus) (aa428-527) - LS-G59859

Source:

Wheat Germ Extract

Tag:

GST, N-terminus

Price:

2 µg/$439

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Human RAG2 / RAG-2 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G79516

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

59.1 kDa

Price:

20 µg/$150

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Human RAG2 / RAG-2 (Sandwich ELISA) ELISA Kit - LS-F12467

Type:

Sandwich

Format:

96-Well Strip Plate

Reactivity:

Human

Range:

0.312 - 20 ng/ml

Detection:

Colorimetric - 450nm (TMB)

Sample Types:

Plasma, Serum

Price:

1 Plate/$783

Viewing 1-25 of 32 product results

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