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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3760-0.05 | 0.05 mg (1 mg/ml) | $460 |
1 of 2
2 of 2
PathPlus™ Monoclonal Rat anti‑Mouse ITGAM / CD11b Antibody (clone M1/70.15, IHC, IF) LS‑B3760
PathPlus™ Monoclonal Rat anti‑Mouse ITGAM / CD11b Antibody (clone M1/70.15, IHC, IF) LS‑B3760
Note: This antibody replaces LS-C45334, LS-C45335
Antibody:
ITGAM / CD11b Rat anti-Mouse Monoclonal (M1/70.15) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF, IP, Flo
Reactivity:
Mouse, Human, Rabbit
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
ITGAM / CD11b Rat anti-Mouse Monoclonal (M1/70.15) Antibody
Application:
IHC, IHC-P, IHC-Fr, IF, IP, Flo
Reactivity:
Mouse, Human, Rabbit
Format:
Unconjugated, Unmodified
Specifications
Description
ITGAM (Integrin alpha-M/beta-2) is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804
Target
Mouse ITGAM / CD11b
Synonyms
ITGAM | Antigen CD11b (p170) | CD11B | CD11b antigen | CR3A | Integrin alpha-M | MAC-1 | MO1A | Neutrophil adherence receptor | MAC1A | SLEB6 | CR-3 alpha chain
Host
Rat
Reactivity
Mouse, Human, Rabbit
(tested or 100% immunogen sequence identity)
Clonality
IgG2b
Monoclonal
Clone
M1/70.15
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Immunogen
T cell enriched splenocytes from B10 mice
Specificity
Recognizes the murine CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1), a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Clone M1/70.15 has been reported to block iC3b binding to its receptor. This product is routinely tested in flow cytometry on mouse peritoneal macrophages.
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- IHC - Frozen
- Immunofluorescence
- Immunoprecipitation
- Flow Cytometry (1:50)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3760 was validated for use in immunohistochemistry on a panel of formalin-fixed, paraffin-embedded (FFPE) mouse tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3760 was determined to be 10 ug/ml.
Presentation
PBS, 0.09% Sodium Azide
Storage
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
About ITGAM / CD11b
Validation
Anti-CD11b antibody IHC of mouse spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-CD11b antibody IHC of mouse spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
See More About...
LSBio Ratings
PathPlus™ ITGAM / CD11b Antibody (clone M1/70.15) for IHC, IF/Immunofluorescence, IP, Flow LS-B3760 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
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Customer Reviews (0)
Featured Products
Species:
Mouse, Human, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Dog, Bovine, Cat, Goat, Pig, Mustelid
Applications:
IHC, IHC - Frozen, Immunoprecipitation, Flow Cytometry
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
