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Anti-GJB2 / CX26 / Connexin 26 Antibody (Cytoplasmic Domain, clone CX-12H10) IHC-plus™ LS-B5511

Note: This antibody replaces LS-C14523


Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

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Mouse Monoclonal [clone CX-12H10] (IgG1,k) to Rat GJB2 / CX26 / Connexin 26
Rat, Human, Mouse
IHC - Paraffin, IHC - Frozen, Western blot, ELISA


Rat GJB2 / CX26 / Connexin 26
Rat, Human, Mouse (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [CX-12H10]
Immunoaffinity purified


IHC - Paraffin (10 µg/ml), IHC - Frozen, Western blot (1 µg/ml), ELISA (0.1 - 1 µg/ml)

Specificity and Use

GJB2 / CX26 / Connexin 26 antibody was raised against synthetic peptide corresponding to a segment of the cytoplasmic loop of rat Connexin-26. (4,5).
Cytoplasmic Domain
Reacts strongly with Connexin-26, which has a predicted molecular weight of 26.5kD. Although the polyclonal anti-Cx26 antibody (feline. No. 71-0500) raised against the same sequence as this monoclonal anti-Cx26 antibody cross-reacts with Cx30, this monoclonal anti-Cx26 appears to exhibit minimal cross-reaction with Cx30 by western blotting. The degree of cross-reaction with Cx30 by IHC is uncertain, but may be influenced by fixation conditions. Rat, mouse and human. Reactivity of this antibody on Western blots has been confirmed by using extracts from mouse liver, mouse brain and rat brain.
Suitable for use in ELISA, Western Blot and Immunohistochemistry. ELISA: 0.1-1 ug/ml. Immunohistochemistry (Frozen): 1-2 ug/ml. Western Blot: 1 ug/ml. Reactivity confirmed using extracts from mouse liver, mouse brain and rat brain.


PBS, pH 7.4, 0.09% sodium azide.
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12months.
For research use only.

About GJB2 / CX26 / Connexin 26

P29033 NM_004004 NP_003995.2

GJB2 Antibody, Connexin 26 Antibody, Connexin-26 Antibody, DFNB1A Antibody, DFNA3 Antibody, DFNB1 Antibody, Gap junction protein beta 2 Antibody, KID Antibody, HID Antibody, PPK Antibody, NSRD1 Antibody, CX26 Antibody, DFNA3A Antibody

GJB2 / CX26 / Connexin 26 is a member of the gap junction protein family. The gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels that facilitate the transfer of ions and small molecules between cells. The gap junction proteins, also known as connexins, purified from fractions of enriched gap junctions from different tissues differ.


Anti-GJB2 antibody IHC of rat intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5511 concentration 10 ug/ml.

Requested From: United States
Date Requested: 9/30/2016

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