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Catalog Number Size Price
LS-B5764-50 50 µg (0.5 mg/ml) $460 
EBI3 / IL-27B Antibody - Anti-EBI3 antibody IHC of human lymphoid-dendritic cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
EBI3 / IL-27B Antibody - Recombinant mouse EBI3/p28 (covalently linked IL-27) protein (lane 1) and mouse bone marrow-derived dendritic cells stimulated with LPS (lane 2) or left untreated (lane3) were analyzed by SDS-PAGE followed by immunoblotting with 5 ug/ml of anti-mouse EBI3 (DNT27). Bands were visualized with horseradish peroxidase (HRP)-conjugated goat anti-rat IgG.
EBI3 / IL-27B Antibody - Anti-EBI3 antibody IHC of human lymphoid-dendritic cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
EBI3 / IL-27B Antibody - Recombinant mouse EBI3/p28 (covalently linked IL-27) protein (lane 1) and mouse bone marrow-derived dendritic cells stimulated with LPS (lane 2) or left untreated (lane3) were analyzed by SDS-PAGE followed by immunoblotting with 5 ug/ml of anti-mouse EBI3 (DNT27). Bands were visualized with horseradish peroxidase (HRP)-conjugated goat anti-rat IgG.
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IHC‑plus™ Monoclonal Rat anti‑Mouse EBI3 / IL‑27B Antibody (clone DNT27, IHC, WB) LS‑B5764

IHC‑plus™ Monoclonal Rat anti‑Mouse EBI3 / IL‑27B Antibody (clone DNT27, IHC, WB) LS‑B5764

Note: This antibody replaces LS-C107123
Antibody:
EBI3 / IL-27B Rat anti-Mouse Monoclonal (DNT27) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B5764-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
EBI3 / IL-27B Rat anti-Mouse Monoclonal (DNT27) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Human
Format:
Unconjugated, Unmodified

Specifications

Description
IL-27B antibody LS-B5764 is an unconjugated rat monoclonal antibody to IL-27B (EBI3) from mouse. It is reactive with human and mouse. Validated for IHC and WB. Cited in 1 publication.
Target
Mouse EBI3 / IL-27B
Synonyms
EBI3 | Epstein-Barr virus induced 3 | IL-27 subunit beta | IL27 subunit | IL27B | Interleukin-27 subunit beta | IL-27B | Cytokine receptor | EBV-induced gene 3 protein | IL35 subunit
Host
Rat
Reactivity
Mouse, Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
DNT27
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Mouse EBI3
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This DNT27 antibody has been tested by western blot of cell lysates prepared from mouse EBI3-transfected 293T cells and bone marrow-derived dendritic cells. This can be used at less than or equal to 1-5 ug/ml. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Presentation
Tissue Culture Supernatant, 0.09% Sodium Azide
Storage
Store at 4°C, avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About EBI3 / IL-27B
Q14213 NM_005755 NP_005746.2

Validation

EBI3 / IL-27B Antibody - Anti-EBI3 antibody IHC of human lymphoid-dendritic cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-EBI3 antibody IHC of human lymphoid-dendritic cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-EBI3 antibody IHC of human lymphoid-dendritic cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ EBI3 / IL-27B Antibody (clone DNT27) for IHC, WB/Western LS-B5764 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

IL-35 mitigates murine acute graft-versus-host disease with retention of graft-versus-leukemia effects. Liu Y, Wu Y, Wang Y, Cai Y, Hu B, Bao G, Fang H, Zhao L, Ma S, Cheng Q, Song Y, Liu Y, Zhu Z, Chang H, Yu X, Sun A, Zhang Y, Vignali DA, Wu D, Liu H. Leukemia. 2015 29:939-46. (IHC; Mouse)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024