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Catalog Number Size Price
LS-A2867-50 50 µg (1 mg/ml) $440 
DKK1 Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
DKK1 Antibody - Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
DKK1 Antibody - Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
DKK1 Antibody - Human Uterus, Endometrium: Formalin-Fixed, Paraffin-Embedded (FFPE)
DKK1 Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
DKK1 Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
DKK1 Antibody - Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
DKK1 Antibody - Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
DKK1 Antibody - Human Uterus, Endometrium: Formalin-Fixed, Paraffin-Embedded (FFPE)
DKK1 Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
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IHC‑plus™ Polyclonal Rabbit anti‑Human DKK1 Antibody (Internal Region, IHC, WB) LS‑A2867

IHC‑plus™ Polyclonal Rabbit anti‑Human DKK1 Antibody (Internal Region, IHC, WB) LS‑A2867

Note: This antibody replaces LS-C64745, LS-C42470
Antibody:
DKK1 Rabbit anti-Human Polyclonal (Internal Region) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$440
LS-A2867-50

Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
DKK1 Rabbit anti-Human Polyclonal (Internal Region) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
DKK1 antibody LS-A2867 is an unconjugated rabbit polyclonal antibody to human DKK1 (Internal Region). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
Target
Human DKK1
Synonyms
DKK1 | Dickkopf-1 like | DKK-1 | Dickkopf-1 | Dickkopf-related protein 1 | Dickkopf related protein-1 | HDkk-1 | Hdkk1 | Dickkopf-like protein 1 | SK
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic 15 amino acid peptide from human DKK1. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon (100%); Monkey (93%); Marmoset (80%).
Epitope
Internal Region
Specificity
Human DKK1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About DKK1
O94907 NM_012242 NP_036374.1

Validation

DKK1 Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

DKK1 Antibody - Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)

DKK1 Antibody - Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)

DKK1 Antibody - Human Uterus, Endometrium: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Uterus, Endometrium: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Uterus, Endometrium: Formalin-Fixed, Paraffin-Embedded (FFPE)

DKK1 Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

LSBio Ratings

IHC-plus™ DKK1 Antibody (Internal Region) for IHC, WB/Western LS-A2867 has an LSBio Rating of
Publications (4.2)
Laboratory Validation Score (4)

Publications (3)

Nitric oxide production upregulates Wnt/ beta -catenin signaling by inhibiting Dickkopf-1. Du Q, Zhang X, Liu Q, Zhang X, Bartels CE, Geller DA. Cancer research. 2013 Nov;73:6526-37.

Wnt antagonist DKK1 acts as a tumor suppressor gene that induces apoptosis and inhibits proliferation in human renal cell carcinoma. Hirata H, Hinoda Y, Nakajima K, Kawamoto K, Kikuno N, Ueno K, Yamamura S, Zaman MS, Khatri G, Chen Y, Saini S, Majid S, Deng G, Ishii N, Dahiya R. International journal of cancer. Journal international du cancer. 2011 Apr;128:1793-803.

Differential expression of Dickkopf-1 among non-small cell lung cancer cells. Xiang XJ, Liu YW, Chen DD, Yu S. Molecular medicine reports. 2015 August;12:1935-1940.

Customer Reviews (0)


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Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024