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| Catalog Number | Size | Price |
|---|---|---|
| LS-B10616-50 | 50 µg | $515 |
1 of 2
2 of 2
IHC‑plus™ Polyclonal Rabbit anti‑Human CTCF Antibody (C‑Terminus, IHC, WB) LS‑B10616
IHC‑plus™ Polyclonal Rabbit anti‑Human CTCF Antibody (C‑Terminus, IHC, WB) LS‑B10616
Note: This antibody replaces LS-C153852
Antibody:
CTCF Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
CTCF Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Specifications
Description
CTCF antibody LS-B10616 is an unconjugated rabbit polyclonal antibody to CTCF (C-Terminus) from human. It is reactive with human and mouse. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human CTCF
Synonyms
CTCF | 11-zinc finger protein | CCCTC-binding factor | Transcriptional repressor CTCF | CTCFL paralog
Host
Rabbit
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Predicted
Mouse, Bovine, Horse, Panda, Chicken (at least 90% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
CTCF affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of CTCF protein.
Epitope
C-Terminus
Specificity
This antibody reacts with endogenous CTCF protein. A BLAST analysis was used to suggest reactivity with CTCF from human, mouse, horse, bovine, panda, rabbit, Danio, and chicken based on a 100% homology with the immunizing sequence. Cross-reactivity with CTCF from other sources has not been determined.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1:500 - 1:2000)
- ELISA (1:10000 - 1:50000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
This affinity purified antibody is suitable for ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 82.8 kD or 150 kD in size corresponding to CTCF by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About CTCF
Validation
Anti-CTCF antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-CTCF antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ CTCF Antibody (C-Terminus) for IHC, WB/Western, ELISA LS-B10616 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Mouse
Applications:
IHC, Western blot, Immunoprecipitation
Species:
Human
Applications:
ICC, Immunofluorescence, Western blot, Dot Blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
Date Requested: 4/18/2024
