Target
Human CDKN1B / p27 Kip1
Synonyms
CDKN1B | KIP1 | MEN1B | P27 Kip1 | p27KIP1 | MEN4 | CDKN4
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Immunogen
This antibody was prepared from whole rabbit serum produced by repeated immunizations with a full length recombinant human p27 protein.
Specificity
Antiserum will specifically react with a p27 protein from human tissue. No reaction was observed against other related tumor suppressor proteins. No reactivity is observed with p27 from mouse. Reactivity against p27 from other species is unknown
Applications
- Western blot (1:1000 - 1:4000)
- Immunoprecipitation (1:100)
- ELISA (1:10000 - 1:40000)
Usage
This antibody is suitable for use in ELISA, immunoprecipitation, coimmunoprecipitation and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~27 kD in size corresponding to p27 by western blotting in the appropriate cell lysate or extract. Anti-p27 is suitable for the detection by immunoblot of human p27. No reactivity is observed with p27 from mouse. This antibody is suitable for immunoprecipitation of in vitro translated protein and cell lysates (C212, 3T3-L1) and will co-precipitate associated proteins. Optimal titers for other applications should be determined by the researcher.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee