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Catalog Number Size Price
LS-C209432-100 100 µg (0.68 mg/ml) $567 
CDKN1B / p27 Kip1 Antibody - Western Blot of Rabbit anti-p27kip1pS140 Antibody. Lane 1: 293 cells untransfected. Lane 2: 293 cells untransfected and treated. Lane 3: 293 cells transfected with HA-p27wt. Lane 4: 293 cells transfected with HA-p27wt and treated. Lane 5: 293 cells transfected with HA-p27S140A. Lane 6: 293 cells transfected with HA-p27S140A and treated. Load: 20 µg per lane. Primary antibody: p27kip1pS140 antibody at 1:250 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10,000 for 45 min at RT.

Polyclonal Rabbit anti‑Human CDKN1B / p27 Kip1 Antibody (phospho‑Ser140, IHC, WB) LS‑C209432

Polyclonal Rabbit anti‑Human CDKN1B / p27 Kip1 Antibody (phospho‑Ser140, IHC, WB) LS‑C209432

Antibody:
CDKN1B / p27 Kip1 Rabbit anti-Human Polyclonal (pSer140) Antibody
Application:
IHC, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C209432-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
CDKN1B / p27 Kip1 Rabbit anti-Human Polyclonal (pSer140) Antibody
Application:
IHC, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
P27 Kip1 antibody LS-C209432 is an unconjugated rabbit polyclonal antibody to human p27 Kip1 (CDKN1B) (pSer140). Validated for ELISA, IHC, IP and WB.
Target
Human CDKN1B / p27 Kip1
Synonyms
CDKN1B | KIP1 | MEN1B | P27 Kip1 | p27KIP1 | MEN4 | CDKN4
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Anti-p27 kip1 pS140 was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to S140 residue of human p27 kip1 protein.
Epitope
pSer140
Applications
  • IHC
  • Western blot (1:500 - 1:4000)
  • Immunoprecipitation
  • ELISA (1:10000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Anti-p27kip1 pS140 antibody is suitable for use in ELISA, immunoprecipitation, co-immunoprecipitation and western blotting. Expect a band at ~27 kD in size corresponding to p27 by western blotting in the appropriate cell lysate or extract. Anti-p27 is suitable for the detection by immunoblot of human p27. Optimal titers for other applications should be determined by the researcher.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About CDKN1B / p27 Kip1
P46527 NM_004064 NP_004055.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024