Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human (tested or 100% immunogen sequence identity)
IHC - Paraffin (5 µg/ml)
Western blot (0.1 - 0.3 µg/ml)
Specificity and Use
BAG3 / BAG-3 antibody was raised against synthetic peptide C-KNAGNAEDPHTE from an internal region of human BAG3 / BAG-3 (NP_004272.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%); Gibbon, Monkey (92%).
Human BAG3 / BAG-3
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 70kD band observed in Human Pancreas lysates (calculated MW of 61.6kD according to NP_004272.2). Recommended concentration: 0.1-0.3 ug/ml.
BAG3 Antibody, Bcl-2-associated athanogene 3 Antibody, Bcl-2-binding protein Bis Antibody, BCL2-binding athanogene 3 Antibody, BIS Antibody, BAG-3 Antibody, CAIR-1 Antibody, Docking protein cair1 Antibody, BCL2-associated athanogene 3 Antibody, Docking protein CAIR-1 Antibody
BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells.